The good, the bad, and the tiny: a review of microflow cytometry

@article{citeulike:2989265, abstract = {Abstract\ \ Recent developments in microflow cytometry have concentrated on advancing technology in four main areas: (1) focusing the particles to be analyzed in the microfluidic channel, (2) miniaturization of the fluid-handling components, (3) miniaturization of the optics, and (4) integration and applications development. Strategies for focusing particles in a narrow path as they pass through the detection region include the use of focusing fluids, nozzles, and dielectrophoresis. Strategies for optics range from the use of microscope objectives to polymer waveguides or optical fibers embedded on-chip. While most investigators use off-chip fluidic control, there are a few examples of integrated valves and pumps. To date, demonstrations of applications are primarily used to establish that the microflow systems provide data of the same quality as laboratory systems, but new capabilities—such as automated sample staining—are beginning to emerge. Each of these four areas is discussed in detail in terms of the progress of development, the continuing limitations, and potential future directions for microflow cytometers.}, author = {Ateya, Daniel and Erickson, Jeffrey and Howell, Peter and Hilliard, Lisa and Golden, Joel and Ligler, Frances }, citeulike-article-id = {2989265}, doi = {http://dx.doi.org/10.1007/s00216-007-1827-5}, journal = {Analytical and Bioanalytical Chemistry}, keywords = {biology, cell, lab-on-a-chip, microfluidics, review}, month = {July}, number = {5}, pages = {1485--1498}, posted-at = {2008-07-11 19:50:17}, priority = {2}, title = {The good, the bad, and the tiny: a review of microflow cytometry}, url = {http://dx.doi.org/10.1007/s00216-007-1827-5}, volume = {391}, year = {2008} }

TY - JOUR ID - citeulike:2989265 L3 - citeulike-article-id:2989265 TI - The good, the bad, and the tiny: a review of microflow cytometry JF - Analytical and Bioanalytical Chemistry VL - 391 IS - 5 SP - 1485 EP - 1498 N2 - Abstract  Recent developments in microflow cytometry have concentrated on advancing technology in four main areas: (1) focusing the particles to be analyzed in the microfluidic channel, (2) miniaturization of the fluid-handling components, (3) miniaturization of the optics, and (4) integration and applications development. Strategies for focusing particles in a narrow path as they pass through the detection region include the use of focusing fluids, nozzles, and dielectrophoresis. Strategies for optics range from the use of microscope objectives to polymer waveguides or optical fibers embedded on-chip. While most investigators use off-chip fluidic control, there are a few examples of integrated valves and pumps. To date, demonstrations of applications are primarily used to establish that the microflow systems provide data of the same quality as laboratory systems, but new capabilities—such as automated sample staining—are beginning to emerge. Each of these four areas is discussed in detail in terms of the progress of development, the continuing limitations, and potential future directions for microflow cytometers. KW - biology KW - cell KW - lab-on-a-chip KW - microfluidics KW - review AU - Ateya, Daniel AU - Erickson, Jeffrey AU - Howell, Peter AU - Hilliard, Lisa AU - Golden, Joel AU - Ligler, Frances PY - 2008/07/01/ UR - http://dx.doi.org/10.1007/s00216-007-1827-5 ER -