PRIMEGENS-v2: Genome-wide primer design for analyzing DNA methylation patterns of CpG islands

@article{citeulike:2972988, abstract = {Motivation: DNA methylation plays important roles in biological processes and human diseases, especially cancers. High-throughput bisulfite genomic sequencing based on new-generation of sequencers, such as the 454-sequencing system provides an efficient method for analyzing DNA methylation patterns. The successful implementation of this approach depends on the use of primer design software capable of performing genome-wide scan for optimal primers from in silico bisulfite-treated genome sequences. We have developed a method, which fulfills this requirement and conduct primer design for sequences including regions of given promoter CpG islands. Results: The developed method has been implemented using the C and JAVA programming languages. The primer design results were tested in the PCR experiments of 96 selected human DNA sequences containing CpG islands in the promoter regions. The results indicate that this method is efficient and reliable for designing sequence-specific primers. Availability: The sequence-specific primer design for DNA methylated sequences including CpG islands has been integrated into the second version of PRIMEGENS as one of the primer design features. The software is freely available for academic use at http://digbio.missouri.edu/primegens/. 10.1093/bioinformatics/btn320}, author = {Srivastava, Gyan P. and Guo, Juyuan and Shi, Huidong and Xu, Dong }, citeulike-article-id = {2972988}, doi = {http://dx.doi.org/10.1093/bioinformatics/btn320}, journal = {Bioinformatics}, keywords = {cpg, dna, methylation, primer}, month = {June}, pages = {btn320+}, posted-at = {2008-07-18 04:54:14}, priority = {2}, title = {PRIMEGENS-v2: Genome-wide primer design for analyzing DNA methylation patterns of CpG islands}, url = {http://dx.doi.org/10.1093/bioinformatics/btn320}, year = {2008} }

TY - JOUR ID - citeulike:2972988 L3 - citeulike-article-id:2972988 TI - PRIMEGENS-v2: Genome-wide primer design for analyzing DNA methylation patterns of CpG islands JF - Bioinformatics SP - btn320 N2 - Motivation: DNA methylation plays important roles in biological processes and human diseases, especially cancers. High-throughput bisulfite genomic sequencing based on new-generation of sequencers, such as the 454-sequencing system provides an efficient method for analyzing DNA methylation patterns. The successful implementation of this approach depends on the use of primer design software capable of performing genome-wide scan for optimal primers from in silico bisulfite-treated genome sequences. We have developed a method, which fulfills this requirement and conduct primer design for sequences including regions of given promoter CpG islands. Results: The developed method has been implemented using the C and JAVA programming languages. The primer design results were tested in the PCR experiments of 96 selected human DNA sequences containing CpG islands in the promoter regions. The results indicate that this method is efficient and reliable for designing sequence-specific primers. Availability: The sequence-specific primer design for DNA methylated sequences including CpG islands has been integrated into the second version of PRIMEGENS as one of the primer design features. The software is freely available for academic use at http://digbio.missouri.edu/primegens/. 10.1093/bioinformatics/btn320 KW - cpg KW - dna KW - methylation KW - primer AU - Srivastava, Gyan AU - Guo, Juyuan AU - Shi, Huidong AU - Xu, Dong PY - 2008/06/25/ UR - http://dx.doi.org/10.1093/bioinformatics/btn320 ER -