The HIV lipidome: A raft with an unusual composition

@article{citeulike:2794914, abstract = {The lipids of enveloped viruses play critical roles in viral morphogenesis and infectivity. They are derived from the host membranes from which virus budding occurs, but the precise lipid composition has not been determined for any virus. Employing mass spectrometry, this study provides a quantitative analysis of the lipid constituents of HIV and a comprehensive comparison with its host membranes. Both a substantial enrichment of the unusual sphingolipid dihydrosphingomyelin and a loss of viral infectivity upon inhibition of sphingolipid biosynthesis in host cells are reported, establishing a critical role for this lipid class in the HIV replication cycle. Intriguingly, the overall lipid composition of native HIV membranes resembles detergent-resistant membrane microdomains and is strikingly different from that of host cell membranes. With this composition, the HIV lipidome provides strong evidence for the existence of lipid rafts in living cells. 10.1073/pnas.0511136103}, author = {Brugger, Britta and Glass, Barbel and Haberkant, Per and Leibrecht, Iris and Wieland, Felix T. and Krausslich, Hans-Georg }, citeulike-article-id = {2794914}, doi = {10.1073/pnas.0511136103}, journal = {Proceedings of the National Academy of Sciences}, keywords = {lipids}, month = {February}, number = {8}, pages = {2641--2646}, posted-at = {2008-05-13 14:06:48}, priority = {2}, title = {The HIV lipidome: A raft with an unusual composition}, url = {http://dx.doi.org/10.1073/pnas.0511136103}, volume = {103}, year = {2006} }

TY - JOUR ID - citeulike:2794914 L3 - citeulike-article-id:2794914 TI - The HIV lipidome: A raft with an unusual composition JF - Proceedings of the National Academy of Sciences VL - 103 IS - 8 SP - 2641 EP - 2646 N2 - The lipids of enveloped viruses play critical roles in viral morphogenesis and infectivity. They are derived from the host membranes from which virus budding occurs, but the precise lipid composition has not been determined for any virus. Employing mass spectrometry, this study provides a quantitative analysis of the lipid constituents of HIV and a comprehensive comparison with its host membranes. Both a substantial enrichment of the unusual sphingolipid dihydrosphingomyelin and a loss of viral infectivity upon inhibition of sphingolipid biosynthesis in host cells are reported, establishing a critical role for this lipid class in the HIV replication cycle. Intriguingly, the overall lipid composition of native HIV membranes resembles detergent-resistant membrane microdomains and is strikingly different from that of host cell membranes. With this composition, the HIV lipidome provides strong evidence for the existence of lipid rafts in living cells. 10.1073/pnas.0511136103 KW - lipids AU - Brugger, Britta AU - Glass, Barbel AU - Haberkant, Per AU - Leibrecht, Iris AU - Wieland, Felix AU - Krausslich, Hans-Georg PY - 2006/02/21/ UR - http://dx.doi.org/10.1073/pnas.0511136103 ER -