Miniaturising the laboratory in emulsion droplets

by: Andrew D Griffiths, Dan S Tawfik

Trends in Biotechnology, Vol. 24, No. 9. (September 2006), pp. 395-402.

View FullText article

DOI, ScienceDirect

Reviews [Write a review of this article]

There are no reviews of this article

Find related articles from these CiteULike users

dchughes, G4ID (group), Genetics-of-Gambling (group), kevinchannon

Find related articles with these CiteULike tags

emulsion-pcr, microfluidics, molecular-biology, self-assembly, synthetic-biology, techniques

Abstract

Biochemical and genetic assays can be both miniaturized and parallelized by compartmentalization in living cells. In vitro compartmentalization (IVC) offers an alternative strategy based on partitioning reactions in water droplets dispersed to form microscopic compartments in water-in-oil emulsions. The cell-like volumes of these compartments (as low as one femtolitre), the ability to freely determine and regulate their content and the large number of compartments (>1010 per millilitre emulsion) have provided the basis for a range of new, ultra-high-throughput, cell-free technologies. This review describes the scope and potential of IVC in areas such as in vitro evolution of proteins and RNAs, cell-free cloning and sequencing, genetics, genomics, and proteomics.

@article{griffiths2007, abstract = {Biochemical and genetic assays can be both miniaturized and parallelized by compartmentalization in living cells. In vitro compartmentalization (IVC) offers an alternative strategy based on partitioning reactions in water droplets dispersed to form microscopic compartments in water-in-oil emulsions. The cell-like volumes of these compartments (as low as one femtolitre), the ability to freely determine and regulate their content and the large number of compartments (>1010 per millilitre emulsion) have provided the basis for a range of new, ultra-high-throughput, cell-free technologies. This review describes the scope and potential of IVC in areas such as in vitro evolution of proteins and RNAs, cell-free cloning and sequencing, genetics, genomics, and proteomics.}, author = {Griffiths, Andrew D. and Tawfik, Dan S. }, citeulike-article-id = {805246}, doi = {10.1016/j.tibtech.2006.06.009}, journal = {Trends in Biotechnology}, keywords = {microfluidics, molecular-biology, self-assembly, synthetic-biology}, month = {September}, number = {9}, pages = {395--402}, posted-at = {2008-03-31 11:47:41}, priority = {2}, title = {Miniaturising the laboratory in emulsion droplets}, url = {http://dx.doi.org/10.1016/j.tibtech.2006.06.009}, volume = {24}, year = {2006} }

RIS record

TY - JOUR ID - griffiths2007 L3 - citeulike-article-id:805246 TI - Miniaturising the laboratory in emulsion droplets JF - Trends in Biotechnology VL - 24 IS - 9 SP - 395 EP - 402 N2 - Biochemical and genetic assays can be both miniaturized and parallelized by compartmentalization in living cells. In vitro compartmentalization (IVC) offers an alternative strategy based on partitioning reactions in water droplets dispersed to form microscopic compartments in water-in-oil emulsions. The cell-like volumes of these compartments (as low as one femtolitre), the ability to freely determine and regulate their content and the large number of compartments (>1010 per millilitre emulsion) have provided the basis for a range of new, ultra-high-throughput, cell-free technologies. This review describes the scope and potential of IVC in areas such as in vitro evolution of proteins and RNAs, cell-free cloning and sequencing, genetics, genomics, and proteomics. KW - microfluidics KW - molecular-biology KW - self-assembly KW - synthetic-biology AU - Griffiths, Andrew AU - Tawfik, Dan PY - 2006/09// UR - http://dx.doi.org/10.1016/j.tibtech.2006.06.009 ER -

RIS BibTeX