An Extended Transcriptional Network for Pluripotency of Embryonic Stem Cells

by: Jonghwan Kim, Jianlin Chu, Xiaohua Shen, Jianlong Wang, Stuart H Orkin

Cell, Vol. 132, No. 6. (21 March 2008), pp. 1049-1061.

View FullText article

DOI, Pubmed, Hubmed, ScienceDirect

Reviews [Write a review of this article]

There are no reviews of this article

Find related articles from these CiteULike users

tmmurali, maralena, jyuh, kengg, inesdesantiago

Find related articles with these CiteULike tags

chip, dataset-transcriptional-regulatory-network, es, genome-wide, network, stemcell, stem-cell, stem-cells, transcription, wide_mapping

Abstract

Summary Much attention has focused on a small set of transcription factors that maintain human or mouse embryonic stem (ES) cells in a pluripotent state. To gain a more complete understanding of the regulatory network that maintains this state, we identified target promoters of nine transcription factors, including somatic cell reprogramming factors (Oct4, Sox2, Klf4, and c-Myc) and others (Nanog, Dax1, Rex1, Zpf281, and Nac1), on a global scale in mouse ES cells. We found that target genes fall into two classes: promoters bound by few factors tend to be inactive or repressed, whereas promoters bound by more than four factors are largely active in the pluripotent state and become repressed upon differentiation. Furthermore, we propose a transcriptional hierarchy for reprogramming factors and broadly distinguish targets of c-Myc versus other factors. Our data provide a resource for exploration of the complex network maintaining pluripotency.

@article{citeulike:2568025, abstract = {Summary Much attention has focused on a small set of transcription factors that maintain human or mouse embryonic stem (ES) cells in a pluripotent state. To gain a more complete understanding of the regulatory network that maintains this state, we identified target promoters of nine transcription factors, including somatic cell reprogramming factors (Oct4, Sox2, Klf4, and c-Myc) and others (Nanog, Dax1, Rex1, Zpf281, and Nac1), on a global scale in mouse ES cells. We found that target genes fall into two classes: promoters bound by few factors tend to be inactive or repressed, whereas promoters bound by more than four factors are largely active in the pluripotent state and become repressed upon differentiation. Furthermore, we propose a transcriptional hierarchy for reprogramming factors and broadly distinguish targets of c-Myc versus other factors. Our data provide a resource for exploration of the complex network maintaining pluripotency.}, author = {Kim, Jonghwan and Chu, Jianlin and Shen, Xiaohua and Wang, Jianlong and Orkin, Stuart H. }, citeulike-article-id = {2568025}, doi = {http://dx.doi.org/10.1016/j.cell.2008.02.039}, journal = {Cell}, keywords = {chip, es, genome-wide}, month = {March}, number = {6}, pages = {1049--1061}, posted-at = {2008-07-24 22:43:13}, priority = {2}, title = {An Extended Transcriptional Network for Pluripotency of Embryonic Stem Cells}, url = {http://dx.doi.org/10.1016/j.cell.2008.02.039}, volume = {132}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2568025 L3 - citeulike-article-id:2568025 TI - An Extended Transcriptional Network for Pluripotency of Embryonic Stem Cells JF - Cell VL - 132 IS - 6 SP - 1049 EP - 1061 N2 - Summary Much attention has focused on a small set of transcription factors that maintain human or mouse embryonic stem (ES) cells in a pluripotent state. To gain a more complete understanding of the regulatory network that maintains this state, we identified target promoters of nine transcription factors, including somatic cell reprogramming factors (Oct4, Sox2, Klf4, and c-Myc) and others (Nanog, Dax1, Rex1, Zpf281, and Nac1), on a global scale in mouse ES cells. We found that target genes fall into two classes: promoters bound by few factors tend to be inactive or repressed, whereas promoters bound by more than four factors are largely active in the pluripotent state and become repressed upon differentiation. Furthermore, we propose a transcriptional hierarchy for reprogramming factors and broadly distinguish targets of c-Myc versus other factors. Our data provide a resource for exploration of the complex network maintaining pluripotency. KW - chip KW - es KW - genome-wide AU - Kim, Jonghwan AU - Chu, Jianlin AU - Shen, Xiaohua AU - Wang, Jianlong AU - Orkin, Stuart PY - 2008/03/21/ UR - http://dx.doi.org/10.1016/j.cell.2008.02.039 ER -

RIS BibTeX