Self-assembling protein microarrays.

@article{citeulike:576377, abstract = {Protein microarrays provide a powerful tool for the study of protein function. However, they are not widely used, in part because of the challenges in producing proteins to spot on the arrays. We generated protein microarrays by printing complementary DNAs onto glass slides and then translating target proteins with mammalian reticulocyte lysate. Epitope tags fused to the proteins allowed them to be immobilized in situ. This obviated the need to purify proteins, avoided protein stability problems during storage, and captured sufficient protein for functional studies. We used the technology to map pairwise interactions among 29 human DNA replication initiation proteins, recapitulate the regulation of Cdt1 binding to select replication proteins, and map its geminin-binding domain.}, address = {Harvard Institute of Proteomics, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Charles Street, Cambridge, MA 02141, USA.}, author = {Ramachandran, N. and Hainsworth, E. and Bhullar, B. and Eisenstein, S. and Rosen, B. and Lau, A. Y. and Walter, J. C. and LaBaer, J. }, citeulike-article-id = {576377}, doi = {http://dx.doi.org/10.1126/science.1097639}, issn = {1095-9203}, journal = {Science}, month = {July}, number = {5680}, pages = {86--90}, posted-at = {2008-01-26 06:54:25}, priority = {2}, title = {Self-assembling protein microarrays.}, url = {http://dx.doi.org/10.1126/science.1097639}, volume = {305}, year = {2004} }

TY - JOUR ID - citeulike:576377 L3 - citeulike-article-id:576377 TI - Self-assembling protein microarrays. JF - Science VL - 305 IS - 5680 SP - 86 EP - 90 AD - Harvard Institute of Proteomics, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Charles Street, Cambridge, MA 02141, USA. SN - 1095-9203 N2 - Protein microarrays provide a powerful tool for the study of protein function. However, they are not widely used, in part because of the challenges in producing proteins to spot on the arrays. We generated protein microarrays by printing complementary DNAs onto glass slides and then translating target proteins with mammalian reticulocyte lysate. Epitope tags fused to the proteins allowed them to be immobilized in situ. This obviated the need to purify proteins, avoided protein stability problems during storage, and captured sufficient protein for functional studies. We used the technology to map pairwise interactions among 29 human DNA replication initiation proteins, recapitulate the regulation of Cdt1 binding to select replication proteins, and map its geminin-binding domain. AU - Ramachandran, N AU - Hainsworth, E AU - Bhullar, B AU - Eisenstein, S AU - Rosen, B AU - Lau, AY AU - Walter, JC AU - LaBaer, J PY - 2004/07/02/ UR - http://dx.doi.org/10.1126/science.1097639 ER -