Aptamer-facilitated biomarker discovery (AptaBiD).

@article{citeulike:3020505, abstract = {Here we introduce a technology for biomarker discovery in which (i) DNA aptamers to biomarkers differentially expressed on the surfaces of cells being in different states are selected; (ii) aptamers are used to isolate biomarkers from the cells; and (iii) the isolated biomarkers are identified by means of mass spectrometry. The technology is termed aptamer-facilitated biomarker discovery (AptaBiD). AptaBiD was used to discover surface biomarkers that distinguish live mature and immature dendritic cells. We selected in vitro two DNA aptamer pools that specifically bind to mature and immature dendritic cells with a difference in strength of approximately 100 times. The aptamer pools were proven to be highly efficient in flow- and magnetic-bead-assisted separation of mature cells from immature cells. The two aptamer pools were then used to isolate biomarkers from the cells. The subsequent mass spectrometry analysis of the isolated proteins revealed unknown biomarkers of immature and mature dendritic cells.}, address = {Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada.}, author = {Berezovski, M. V. and Lechmann, M. and Musheev, M. U. and Mak, T. W. and Krylov, S. N. }, citeulike-article-id = {3020505}, doi = {10.1021/ja801951p}, issn = {1520-5126}, journal = {Journal of the American Chemical Society}, keywords = {aptamer, biomarker}, month = {July}, number = {28}, pages = {9137--9143}, posted-at = {2008-07-19 05:04:02}, priority = {2}, title = {Aptamer-facilitated biomarker discovery (AptaBiD).}, url = {http://dx.doi.org/10.1021/ja801951p}, volume = {130}, year = {2008} }

TY - JOUR ID - citeulike:3020505 L3 - citeulike-article-id:3020505 TI - Aptamer-facilitated biomarker discovery (AptaBiD). JF - Journal of the American Chemical Society VL - 130 IS - 28 SP - 9137 EP - 9143 AD - Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada. SN - 1520-5126 N2 - Here we introduce a technology for biomarker discovery in which (i) DNA aptamers to biomarkers differentially expressed on the surfaces of cells being in different states are selected; (ii) aptamers are used to isolate biomarkers from the cells; and (iii) the isolated biomarkers are identified by means of mass spectrometry. The technology is termed aptamer-facilitated biomarker discovery (AptaBiD). AptaBiD was used to discover surface biomarkers that distinguish live mature and immature dendritic cells. We selected in vitro two DNA aptamer pools that specifically bind to mature and immature dendritic cells with a difference in strength of approximately 100 times. The aptamer pools were proven to be highly efficient in flow- and magnetic-bead-assisted separation of mature cells from immature cells. The two aptamer pools were then used to isolate biomarkers from the cells. The subsequent mass spectrometry analysis of the isolated proteins revealed unknown biomarkers of immature and mature dendritic cells. KW - aptamer KW - biomarker AU - Berezovski, MV AU - Lechmann, M AU - Musheev, MU AU - Mak, TW AU - Krylov, SN PY - 2008/07/16/ UR - http://dx.doi.org/10.1021/ja801951p ER -