Analyzing genes using closing and replicating circles

by: Mats Nilsson, Fredrik Dahl, Chatarina Larsson, Mats Gullberg, Johan Stenberg

Trends in Biotechnology, Vol. 24, No. 2. (February 2006), pp. 83-88.

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Abstract

During the past two years, significant breakthroughs have been achieved in genetic analyses through the application of technologies based on analytical DNA-circularization reactions. Padlock probes and molecular inversion probes have enabled parallel, high-throughput single nucleotide polymorphism (SNP) genotyping at increased scales, whereas, at the other end of the analysis spectrum, DNA molecules in individual cells have been genotyped, in situ, using padlock probes and rolling-circle amplification (RCA). This review describes the recent developments in the technologies that use specific DNA circularization, coupled to DNA amplification through PCR or rolling-circle amplification, and addresses the great potential of these tools.

@article{citeulike:3014410, abstract = {During the past two years, significant breakthroughs have been achieved in genetic analyses through the application of technologies based on analytical DNA-circularization reactions. Padlock probes and molecular inversion probes have enabled parallel, high-throughput single nucleotide polymorphism (SNP) genotyping at increased scales, whereas, at the other end of the analysis spectrum, DNA molecules in individual cells have been genotyped, in situ, using padlock probes and rolling-circle amplification (RCA). This review describes the recent developments in the technologies that use specific DNA circularization, coupled to DNA amplification through PCR or rolling-circle amplification, and addresses the great potential of these tools.}, author = {Nilsson, Mats and Dahl, Fredrik and Larsson, Chatarina and Gullberg, Mats and Stenberg, Johan }, citeulike-article-id = {3014410}, doi = {http://dx.doi.org/10.1016/j.tibtech.2005.12.005}, journal = {Trends in Biotechnology}, keywords = {rca}, month = {February}, number = {2}, pages = {83--88}, posted-at = {2008-07-17 15:14:12}, priority = {2}, title = {Analyzing genes using closing and replicating circles}, url = {http://dx.doi.org/10.1016/j.tibtech.2005.12.005}, volume = {24}, year = {2006} }

RIS record

TY - JOUR ID - citeulike:3014410 L3 - citeulike-article-id:3014410 TI - Analyzing genes using closing and replicating circles JF - Trends in Biotechnology VL - 24 IS - 2 SP - 83 EP - 88 N2 - During the past two years, significant breakthroughs have been achieved in genetic analyses through the application of technologies based on analytical DNA-circularization reactions. Padlock probes and molecular inversion probes have enabled parallel, high-throughput single nucleotide polymorphism (SNP) genotyping at increased scales, whereas, at the other end of the analysis spectrum, DNA molecules in individual cells have been genotyped, in situ, using padlock probes and rolling-circle amplification (RCA). This review describes the recent developments in the technologies that use specific DNA circularization, coupled to DNA amplification through PCR or rolling-circle amplification, and addresses the great potential of these tools. KW - rca AU - Nilsson, Mats AU - Dahl, Fredrik AU - Larsson, Chatarina AU - Gullberg, Mats AU - Stenberg, Johan PY - 2006/02// UR - http://dx.doi.org/10.1016/j.tibtech.2005.12.005 ER -

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