An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications.

@article{citeulike:2793895, abstract = {RNA interference (RNAi) has become a powerful tool for investigating gene function, and, in addition, shows potential for the development of therapeutic agents. RNAi can be triggered in a variety of eukaryotic cells using small interfering RNA (siRNA), their double-stranded precursors (double-stranded RNA) and short hairpin precursors (shRNA). Here, we describe a protocol for analyzing these RNAs and their modifications using electrospray ionization mass spectrometry (ESI-MS). This protocol involves the desalting of nucleic acids using ammonium acetate precipitation, followed by characterization using ESI-MS. This protocol has been chiefly used for analyzing siRNAs and their chemical modifications, but it has also been used and can be applied to the analysis of a wide range of native and modified oligonucleotides. This protocol provides accurate information on molecular weight for a range of nucleic acids and can be completed in less than a day.}, address = {Division of Pharmaceutical Sciences, 5005 Rockhill Road, University of Missouri, Kansas City, Missouri 64110, USA.}, author = {Shah, S. and Friedman, S. H. }, citeulike-article-id = {2793895}, doi = {10.1038/nprot.2007.535}, issn = {1750-2799}, journal = {Nature protocols}, number = {3}, pages = {351--356}, posted-at = {2008-05-13 09:16:33}, priority = {2}, title = {An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications.}, url = {http://dx.doi.org/10.1038/nprot.2007.535}, volume = {3}, year = {2008} }

TY - JOUR ID - citeulike:2793895 L3 - citeulike-article-id:2793895 TI - An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications. JF - Nature protocols VL - 3 IS - 3 SP - 351 EP - 356 AD - Division of Pharmaceutical Sciences, 5005 Rockhill Road, University of Missouri, Kansas City, Missouri 64110, USA. SN - 1750-2799 N2 - RNA interference (RNAi) has become a powerful tool for investigating gene function, and, in addition, shows potential for the development of therapeutic agents. RNAi can be triggered in a variety of eukaryotic cells using small interfering RNA (siRNA), their double-stranded precursors (double-stranded RNA) and short hairpin precursors (shRNA). Here, we describe a protocol for analyzing these RNAs and their modifications using electrospray ionization mass spectrometry (ESI-MS). This protocol involves the desalting of nucleic acids using ammonium acetate precipitation, followed by characterization using ESI-MS. This protocol has been chiefly used for analyzing siRNAs and their chemical modifications, but it has also been used and can be applied to the analysis of a wide range of native and modified oligonucleotides. This protocol provides accurate information on molecular weight for a range of nucleic acids and can be completed in less than a day. AU - Shah, S AU - Friedman, SH PY - 2008/// UR - http://dx.doi.org/10.1038/nprot.2007.535 ER -