Proteomic identification of palmitoylated proteins.

by: AF Roth, J Wan, WN Green, JR Yates, NG Davis

Methods (San Diego, Calif.), Vol. 40, No. 2. (October 2006), pp. 135-142.

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Abstract

A proteomic method that purifies and identifies palmitoylated proteins from complex protein extracts is described. Using the fatty acid exchange labeling chemistry (described in the preceding report), palmitoyl modifications are exchanged for biotinylated compounds, allowing the subset of palmitoyl-proteins to be affinity-purified and then identified by mass spectroscopic protein identification technologies. The advantages and pitfalls of this new technology are discussed within the context of the recent application of this method in the yeast Saccharomyces cerevisiae.

@article{citeulike:2784517, abstract = {A proteomic method that purifies and identifies palmitoylated proteins from complex protein extracts is described. Using the fatty acid exchange labeling chemistry (described in the preceding report), palmitoyl modifications are exchanged for biotinylated compounds, allowing the subset of palmitoyl-proteins to be affinity-purified and then identified by mass spectroscopic protein identification technologies. The advantages and pitfalls of this new technology are discussed within the context of the recent application of this method in the yeast Saccharomyces cerevisiae.}, address = {Department of Pharmacology, Wayne State University School of Medicine, Elliman Building, Room 1224, 421 E. Canfield, Detroit, MI 48201, USA.}, author = {Roth, A. F. and Wan, J. and Green, W. N. and Yates, J. R. and Davis, N. G. }, citeulike-article-id = {2784517}, doi = {10.1016/j.ymeth.2006.05.026}, issn = {1046-2023}, journal = {Methods (San Diego, Calif.)}, month = {October}, number = {2}, pages = {135--142}, posted-at = {2008-05-11 15:46:48}, priority = {2}, title = {Proteomic identification of palmitoylated proteins.}, url = {http://dx.doi.org/10.1016/j.ymeth.2006.05.026}, volume = {40}, year = {2006} }

RIS record

TY - JOUR ID - citeulike:2784517 TI - Proteomic identification of palmitoylated proteins. JF - Methods (San Diego, Calif.) VL - 40 IS - 2 SP - 135 EP - 142 AD - Department of Pharmacology, Wayne State University School of Medicine, Elliman Building, Room 1224, 421 E. Canfield, Detroit, MI 48201, USA. SN - 1046-2023 N2 - A proteomic method that purifies and identifies palmitoylated proteins from complex protein extracts is described. Using the fatty acid exchange labeling chemistry (described in the preceding report), palmitoyl modifications are exchanged for biotinylated compounds, allowing the subset of palmitoyl-proteins to be affinity-purified and then identified by mass spectroscopic protein identification technologies. The advantages and pitfalls of this new technology are discussed within the context of the recent application of this method in the yeast Saccharomyces cerevisiae. AU - Roth, AF AU - Wan, J AU - Green, WN AU - Yates, JR AU - Davis, NG PY - 2006/10// UR - http://dx.doi.org/10.1016/j.ymeth.2006.05.026 ER -

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