Apoptosis-associated mitochondrial outer membrane permeabilization assays.

@article{citeulike:2784504, abstract = {Following most cell death signals, pro-apoptotic Bcl-2 members as Bax and Bak are activated and oligomerize into the mitochondria outer membrane, triggering its permeabilization and release into the cytosol of soluble apoptogenic factors such as cytochrome c involved in caspase activation. Thus, in many studies focused on apoptosis, cytochrome c release within cells is frequently examined to assess Bax/Bak activation and mitochondrial outer membrane permeabilization. In addition, cytochrome c release can also be investigated in vitro in functional mitochondria that have been isolated from cultured cells, offering a number of advantages. Here, protocols for measuring cytochrome c release from intact cells as well as from isolated mitochondria is detailed. Finally, assays to investigate Bax/Bak activation and olimerization are also presented.}, address = {INSERM U 542, Universit\'{e} Paris-Sud, Hopital Paul Brousse, Batiment Lavoisier, 14 avenue Paul Vaillant Couturier, 94807 Villejuif cedex, France. damarn@vjf.inserm.fr}, author = {Arnoult, D. }, citeulike-article-id = {2784504}, doi = {10.1016/j.ymeth.2007.11.003}, issn = {1046-2023}, journal = {Methods (San Diego, Calif.)}, month = {March}, number = {3}, pages = {229--234}, posted-at = {2008-05-11 15:40:57}, priority = {2}, title = {Apoptosis-associated mitochondrial outer membrane permeabilization assays.}, url = {http://dx.doi.org/10.1016/j.ymeth.2007.11.003}, volume = {44}, year = {2008} }

TY - JOUR ID - citeulike:2784504 TI - Apoptosis-associated mitochondrial outer membrane permeabilization assays. JF - Methods (San Diego, Calif.) VL - 44 IS - 3 SP - 229 EP - 234 AD - INSERM U 542, Université Paris-Sud, Hopital Paul Brousse, Batiment Lavoisier, 14 avenue Paul Vaillant Couturier, 94807 Villejuif cedex, France. damarn@vjf.inserm.fr SN - 1046-2023 N2 - Following most cell death signals, pro-apoptotic Bcl-2 members as Bax and Bak are activated and oligomerize into the mitochondria outer membrane, triggering its permeabilization and release into the cytosol of soluble apoptogenic factors such as cytochrome c involved in caspase activation. Thus, in many studies focused on apoptosis, cytochrome c release within cells is frequently examined to assess Bax/Bak activation and mitochondrial outer membrane permeabilization. In addition, cytochrome c release can also be investigated in vitro in functional mitochondria that have been isolated from cultured cells, offering a number of advantages. Here, protocols for measuring cytochrome c release from intact cells as well as from isolated mitochondria is detailed. Finally, assays to investigate Bax/Bak activation and olimerization are also presented. AU - Arnoult, D PY - 2008/03// UR - http://dx.doi.org/10.1016/j.ymeth.2007.11.003 ER -