Characterization of a putative intrarenal serotonergic system.

@article{citeulike:2724232, abstract = {Serotonin [5-hydroxytryptamine (5HT)] acts through multiple G protein-coupled 5-HT receptors, and its activity is also regulated by the 5-HT transporter. The current studies report the expression and localization of the 5-HT receptors and transporter in the kidney. In addition, the enzymatic pathway mediating 5-HT synthesis is present in renal cortex, especially in the proximal tubules and glomerular epithelial cells and mesangial cells. Expression of the 5-HT receptors and 5-HT transporter was detected by RT-PCR in cell lines of these cell types. In cultured proximal tubule cells and podocytes, 5-HT activated ERK1/2 and increased the expression of connective tissue growth factor and transforming growth factor-beta, two key mediators of extracellular matrix accumulation. Immunohistochemistry and real-time RT-PCR studies also indicated that 5-HT stimulated expression of vascular endothelial growth factor in podocytes in vitro and in vivo. Therefore, these results indicate the presence of an integrated intrarenal serotonergic system and suggest a possible role for 5-HT as a mediator of renal fibrosis in the kidney.}, address = {Division of Nephrology, Vanderbilt Univ. School of Medicine, MCN C3121, 1161 21st St. S., Nashville, TN 37232, USA.}, author = {Xu, J. and Yao, B. and Fan, X. and Langworthy, M. M. and Zhang, M. Z. and Harris, R. C. }, citeulike-article-id = {2724232}, doi = {http://dx.doi.org/10.1152/ajprenal.00246.2007}, issn = {0363-6127}, journal = {American journal of physiology. Renal physiology}, month = {November}, number = {5}, posted-at = {2008-04-27 17:33:24}, priority = {2}, title = {Characterization of a putative intrarenal serotonergic system.}, url = {http://dx.doi.org/10.1152/ajprenal.00246.2007}, volume = {293}, year = {2007} }

TY - JOUR ID - citeulike:2724232 L3 - citeulike-article-id:2724232 TI - Characterization of a putative intrarenal serotonergic system. JF - American journal of physiology. Renal physiology VL - 293 IS - 5 AD - Division of Nephrology, Vanderbilt Univ. School of Medicine, MCN C3121, 1161 21st St. S., Nashville, TN 37232, USA. SN - 0363-6127 N2 - Serotonin [5-hydroxytryptamine (5HT)] acts through multiple G protein-coupled 5-HT receptors, and its activity is also regulated by the 5-HT transporter. The current studies report the expression and localization of the 5-HT receptors and transporter in the kidney. In addition, the enzymatic pathway mediating 5-HT synthesis is present in renal cortex, especially in the proximal tubules and glomerular epithelial cells and mesangial cells. Expression of the 5-HT receptors and 5-HT transporter was detected by RT-PCR in cell lines of these cell types. In cultured proximal tubule cells and podocytes, 5-HT activated ERK1/2 and increased the expression of connective tissue growth factor and transforming growth factor-beta, two key mediators of extracellular matrix accumulation. Immunohistochemistry and real-time RT-PCR studies also indicated that 5-HT stimulated expression of vascular endothelial growth factor in podocytes in vitro and in vivo. Therefore, these results indicate the presence of an integrated intrarenal serotonergic system and suggest a possible role for 5-HT as a mediator of renal fibrosis in the kidney. AU - Xu, J AU - Yao, B AU - Fan, X AU - Langworthy, MM AU - Zhang, MZ AU - Harris, RC PY - 2007/11// UR - http://dx.doi.org/10.1152/ajprenal.00246.2007 ER -