Robust hepatitis C virus infection in vitro

by: Jin Zhong, Pablo Gastaminza, Guofeng Cheng, Sharookh Kapadia, Takanobu Kato, Dennis R Burton, Stefan F Wieland, Susan L Uprichard, Takaji Wakita, Francis V Chisari

Proceedings of the National Academy of Sciences, Vol. 102, No. 26. (28 June 2005), pp. 9294-9299.

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Abstract

The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen. 10.1073/pnas.0503596102

@article{citeulike:2604973, abstract = {The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen. 10.1073/pnas.0503596102}, author = {Zhong, Jin and Gastaminza, Pablo and Cheng, Guofeng and Kapadia, Sharookh and Kato, Takanobu and Burton, Dennis R. and Wieland, Stefan F. and Uprichard, Susan L. and Wakita, Takaji and Chisari, Francis V. }, citeulike-article-id = {2604973}, doi = {10.1073/pnas.0503596102}, journal = {Proceedings of the National Academy of Sciences}, month = {June}, number = {26}, pages = {9294--9299}, posted-at = {2008-03-28 04:52:25}, priority = {2}, title = {Robust hepatitis C virus infection in vitro}, url = {http://dx.doi.org/10.1073/pnas.0503596102}, volume = {102}, year = {2005} }

RIS record

TY - JOUR ID - citeulike:2604973 L3 - citeulike-article-id:2604973 TI - Robust hepatitis C virus infection in vitro JF - Proceedings of the National Academy of Sciences VL - 102 IS - 26 SP - 9294 EP - 9299 N2 - The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen. 10.1073/pnas.0503596102 AU - Zhong, Jin AU - Gastaminza, Pablo AU - Cheng, Guofeng AU - Kapadia, Sharookh AU - Kato, Takanobu AU - Burton, Dennis AU - Wieland, Stefan AU - Uprichard, Susan AU - Wakita, Takaji AU - Chisari, Francis PY - 2005/06/28/ UR - http://dx.doi.org/10.1073/pnas.0503596102 ER -

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