Proteomic profiling using mass spectrometry--does normalising by total ion current potentially mask some biological differences?

@article{citeulike:2535339, abstract = {Normalisation of data to minimise the impact of technical variation on comparative sample analysis is often carried out. Using SELDI data as a model, we have examined the effects of normalisation by TIC which is commonly used for MS data. Significant intergroup differences in normalisation factor were found for serum profiles which could not be explained by experimental factors, implying that normalisation by TIC may in some situations also normalise biological differences and should be systematically evaluated.}, address = {Clinical and Biomedical Proteomics Group, Cancer Research UK Clinical Centre, Leeds Institute of Molecular Medicine, St. James's University Hospital, Leeds, UK.}, author = {Cairns, D. A. and Thompson, D. and Perkins, D. N. and Stanley, A. J. and Selby, P. J. and Banks, R. E. }, citeulike-article-id = {2535339}, doi = {http://dx.doi.org/10.1002/pmic.200700598}, issn = {1615-9853}, journal = {Proteomics}, month = {January}, number = {1}, pages = {21--27}, posted-at = {2008-03-15 03:29:56}, priority = {2}, title = {Proteomic profiling using mass spectrometry--does normalising by total ion current potentially mask some biological differences?}, url = {http://dx.doi.org/10.1002/pmic.200700598}, volume = {8}, year = {2008} }

TY - JOUR ID - citeulike:2535339 L3 - citeulike-article-id:2535339 TI - Proteomic profiling using mass spectrometry--does normalising by total ion current potentially mask some biological differences? JF - Proteomics VL - 8 IS - 1 SP - 21 EP - 27 AD - Clinical and Biomedical Proteomics Group, Cancer Research UK Clinical Centre, Leeds Institute of Molecular Medicine, St. James's University Hospital, Leeds, UK. SN - 1615-9853 N2 - Normalisation of data to minimise the impact of technical variation on comparative sample analysis is often carried out. Using SELDI data as a model, we have examined the effects of normalisation by TIC which is commonly used for MS data. Significant intergroup differences in normalisation factor were found for serum profiles which could not be explained by experimental factors, implying that normalisation by TIC may in some situations also normalise biological differences and should be systematically evaluated. AU - Cairns, DA AU - Thompson, D AU - Perkins, DN AU - Stanley, AJ AU - Selby, PJ AU - Banks, RE PY - 2008/01// UR - http://dx.doi.org/10.1002/pmic.200700598 ER -