Aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes.

@article{citeulike:2528201, abstract = {We here report on the first example of an aptamer-based potentiometric sandwich assay of proteins. The measurements are based on CdS quantum dot labels of the secondary aptamer, which were determined with a novel solid-contact Cd2+-selective polymer membrane electrode after dissolution with hydrogen peroxide. The electrode exhibited cadmium ion detection limits of 100 pM in 100 mL samples and of 1 nM in 200 microL microwells, using a calcium-selective electrode as a pseudoreference electrode. As a prototype example, thrombin was measured in 200 microL samples with a lower detection limit of 0.14 nM corresponding to 28 fmol of analyte. The results show great promise for the potentiometric determination of proteins at very low concentrations in microliter samples.}, address = {Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.}, author = {Numnuam, A. and Chumbimuni-Torres, K. Y. and Xiang, Y. and Bash, R. and Thavarungkul, P. and Kanatharana, P. and Pretsch, E. and Wang, J. and Bakker, E. }, citeulike-article-id = {2528201}, doi = {http://dx.doi.org/10.1021/ac701910r}, issn = {0003-2700}, journal = {Anal Chem}, month = {February}, number = {3}, pages = {707--712}, posted-at = {2008-03-13 16:31:30}, priority = {2}, title = {Aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes.}, url = {http://dx.doi.org/10.1021/ac701910r}, volume = {80}, year = {2008} }

TY - JOUR ID - citeulike:2528201 L3 - citeulike-article-id:2528201 TI - Aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes. JF - Anal Chem VL - 80 IS - 3 SP - 707 EP - 712 AD - Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA. SN - 0003-2700 N2 - We here report on the first example of an aptamer-based potentiometric sandwich assay of proteins. The measurements are based on CdS quantum dot labels of the secondary aptamer, which were determined with a novel solid-contact Cd2+-selective polymer membrane electrode after dissolution with hydrogen peroxide. The electrode exhibited cadmium ion detection limits of 100 pM in 100 mL samples and of 1 nM in 200 microL microwells, using a calcium-selective electrode as a pseudoreference electrode. As a prototype example, thrombin was measured in 200 microL samples with a lower detection limit of 0.14 nM corresponding to 28 fmol of analyte. The results show great promise for the potentiometric determination of proteins at very low concentrations in microliter samples. AU - Numnuam, A AU - Chumbimuni-Torres, KY AU - Xiang, Y AU - Bash, R AU - Thavarungkul, P AU - Kanatharana, P AU - Pretsch, E AU - Wang, J AU - Bakker, E PY - 2008/02/01/ UR - http://dx.doi.org/10.1021/ac701910r ER -