Molecular cloning of rat klotho cDNA: markedly decreased expression of klotho by acute inflammatory stress.

@article{citeulike:2452268, abstract = {We have recently identified a novel gene, termed klotho, that is involved in the suppression of several aging phenotypes. The gene encodes a membrane protein that shares sequence similarity with the beta-glucosidases of bacteria and plants. In this study, we isolated rat klotho cDNA and examined its tissue distribution in rats. The deduced amino acid sequence of rat Klotho protein was 1014 amino acids in length and 94 and 85\% homologous to those of mouse and human Klotho proteins, respectively. Northern blot analysis using the rat klotho cDNA probe identified a single transcript of 5.2 kb in size expressed predominantly in the kidney, while RT-PCR detected low levels of expression also in the brain, lung, intestine, and ovaries. During development, klotho expression in the kidney was markedly augmented after birth. Chromosomal localization of rat klotho was mapped to 12q12. Northern blot analysis showed that expression of klotho was markedly decreased by lipopolysaccharide (LPS) in vivo, suggesting that expression of klotho is affected by acute inflammatory stress. The present study leads to a better understanding of the physiologic and pathophysiologic roles of Klotho.}, address = {Second Department of Internal Medicine, Gunma University School of Medicine, 3-39-22, Showa, Gunma, Maebashi, 371-8511, Japan.}, author = {Ohyama, Y. and Kurabayashi, M. and Masuda, H. and Nakamura, T. and Aihara, Y. and Kaname, T. and Suga, T. and Arai, M. and Aizawa, H. and Matsumura, Y. and Kuro-o, M. and Nabeshima, Y. and Nagail, R. }, citeulike-article-id = {2452268}, issn = {0006-291X}, journal = {Biochem Biophys Res Commun}, month = {October}, number = {3}, pages = {920--925}, posted-at = {2008-03-01 16:26:30}, priority = {2}, title = {Molecular cloning of rat klotho cDNA: markedly decreased expression of klotho by acute inflammatory stress.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9791011}, volume = {251}, year = {1998} }

TY - JOUR ID - citeulike:2452268 L3 - citeulike-article-id:2452268 TI - Molecular cloning of rat klotho cDNA: markedly decreased expression of klotho by acute inflammatory stress. JF - Biochem Biophys Res Commun VL - 251 IS - 3 SP - 920 EP - 925 AD - Second Department of Internal Medicine, Gunma University School of Medicine, 3-39-22, Showa, Gunma, Maebashi, 371-8511, Japan. SN - 0006-291X N2 - We have recently identified a novel gene, termed klotho, that is involved in the suppression of several aging phenotypes. The gene encodes a membrane protein that shares sequence similarity with the beta-glucosidases of bacteria and plants. In this study, we isolated rat klotho cDNA and examined its tissue distribution in rats. The deduced amino acid sequence of rat Klotho protein was 1014 amino acids in length and 94 and 85% homologous to those of mouse and human Klotho proteins, respectively. Northern blot analysis using the rat klotho cDNA probe identified a single transcript of 5.2 kb in size expressed predominantly in the kidney, while RT-PCR detected low levels of expression also in the brain, lung, intestine, and ovaries. During development, klotho expression in the kidney was markedly augmented after birth. Chromosomal localization of rat klotho was mapped to 12q12. Northern blot analysis showed that expression of klotho was markedly decreased by lipopolysaccharide (LPS) in vivo, suggesting that expression of klotho is affected by acute inflammatory stress. The present study leads to a better understanding of the physiologic and pathophysiologic roles of Klotho. AU - Ohyama, Y AU - Kurabayashi, M AU - Masuda, H AU - Nakamura, T AU - Aihara, Y AU - Kaname, T AU - Suga, T AU - Arai, M AU - Aizawa, H AU - Matsumura, Y AU - Kuro-o, M AU - Nabeshima, Y AU - Nagail, R PY - 1998/10/29/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9791011 ER -