The human estrogen receptor has two independent nonacidic transcriptional activation functions.

@article{citeulike:2327893, abstract = {plasmid hEG0: We have previously reported the presence of a hormone-inducible transcriptional activation function (TAF-2) within the region of the estrogen receptor (ER) that contains the hormone binding domain. We show here that the N-terminal A/B region of the ER contains an independent constitutive activation function (TAF-1) that exhibits cell type specificity since it activates transcription efficiently in chicken embryo fibroblasts, but only poorly in HeLa cells. By analyzing the ability of TAF-1, TAF-2, and the GAL4 and VP16 acidic activating domains (AADs) to homosynergize and heterosynergize with one another and with the factor binding to the upstream element (UE) of the adenovirus 2 major late promoter, we show that the activation properties of TAF-1 and TAF-2 are different and distinct from those of AADs, in agreement with the absence of acidic amino acid stretches in TAF-1 and TAF-2.}, address = {Laboratoire de G\'{e}n\'{e}tique Mol\'{e}culaire des Eucaryotes du CNRS, Unit\'{e} 184 de Biologie Mol\'{e}culaire et de G\'{e}nie G\'{e}n\'{e}tique de l'INSERM Institut de Chimie Biologique, Facult\'{e} de M\'{e}decine, Strasbourg, France.}, author = {Tora, L. and White, J. and Brou, C. and Tasset, D. and Webster, N. and Scheer, E. and Chambon, P. }, citeulike-article-id = {2327893}, issn = {0092-8674}, journal = {Cell}, keywords = {plasmid}, month = {November}, number = {3}, pages = {477--487}, posted-at = {2008-02-04 02:01:04}, priority = {2}, title = {The human estrogen receptor has two independent nonacidic transcriptional activation functions.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2805068}, volume = {59}, year = {1989} }

TY - JOUR ID - citeulike:2327893 L3 - citeulike-article-id:2327893 TI - The human estrogen receptor has two independent nonacidic transcriptional activation functions. JF - Cell VL - 59 IS - 3 SP - 477 EP - 487 AD - Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France. SN - 0092-8674 N2 - plasmid hEG0: We have previously reported the presence of a hormone-inducible transcriptional activation function (TAF-2) within the region of the estrogen receptor (ER) that contains the hormone binding domain. We show here that the N-terminal A/B region of the ER contains an independent constitutive activation function (TAF-1) that exhibits cell type specificity since it activates transcription efficiently in chicken embryo fibroblasts, but only poorly in HeLa cells. By analyzing the ability of TAF-1, TAF-2, and the GAL4 and VP16 acidic activating domains (AADs) to homosynergize and heterosynergize with one another and with the factor binding to the upstream element (UE) of the adenovirus 2 major late promoter, we show that the activation properties of TAF-1 and TAF-2 are different and distinct from those of AADs, in agreement with the absence of acidic amino acid stretches in TAF-1 and TAF-2. KW - plasmid AU - Tora, L AU - White, J AU - Brou, C AU - Tasset, D AU - Webster, N AU - Scheer, E AU - Chambon, P PY - 1989/11/03/ UR - http://view.ncbi.nlm.nih.gov/pubmed/2805068 ER -