A semiautomated, 96-well plate assay for collagen synthesis.

by: A Scutt, A Berg, H Mayer

Anal Biochem, Vol. 203, No. 2. (June 1992), pp. 290-294.

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Abstract

We have established a rapid method for the measurement of collagen synthesis in large numbers of cell cultures. 3H-labeled collagen in microwell cultures was salt precipitated, harvested, and washed using a commercially available cell harvester and the filtered collagen was directly counted. The cell number could then be assessed either by methylene blue staining or by dissolving the cells in NaOH and estimating the protein content with bicinchoninic acid. In all of these procedures, the samples remain in microtiter plates, thus ensuring that minimal amounts of reagents are used and minimizing the amount of manipulation necessary. The intergroup variability is 3 to 9% and the recovery of 3H-labeled collagen is greater than 90%. Using this method, large numbers of samples can be assessed for collagen synthesis quickly, conveniently, and for minimal cost.

@article{citeulike:2301319, abstract = {We have established a rapid method for the measurement of collagen synthesis in large numbers of cell cultures. 3H-labeled collagen in microwell cultures was salt precipitated, harvested, and washed using a commercially available cell harvester and the filtered collagen was directly counted. The cell number could then be assessed either by methylene blue staining or by dissolving the cells in NaOH and estimating the protein content with bicinchoninic acid. In all of these procedures, the samples remain in microtiter plates, thus ensuring that minimal amounts of reagents are used and minimizing the amount of manipulation necessary. The intergroup variability is 3 to 9\% and the recovery of 3H-labeled collagen is greater than 90\%. Using this method, large numbers of samples can be assessed for collagen synthesis quickly, conveniently, and for minimal cost.}, address = {Gesellschaft f\"{u}r Biotechnologische Forschung mbH, Abteilung Genetik, Braunschweig, Germany.}, author = {Scutt, A. and Berg, A. and Mayer, H. }, citeulike-article-id = {2301319}, issn = {0003-2697}, journal = {Anal Biochem}, keywords = {method}, month = {June}, number = {2}, pages = {290--294}, posted-at = {2008-01-29 09:06:16}, priority = {2}, title = {A semiautomated, 96-well plate assay for collagen synthesis.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1384387}, volume = {203}, year = {1992} }

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TY - JOUR ID - citeulike:2301319 L3 - citeulike-article-id:2301319 TI - A semiautomated, 96-well plate assay for collagen synthesis. JF - Anal Biochem VL - 203 IS - 2 SP - 290 EP - 294 AD - Gesellschaft für Biotechnologische Forschung mbH, Abteilung Genetik, Braunschweig, Germany. SN - 0003-2697 N2 - We have established a rapid method for the measurement of collagen synthesis in large numbers of cell cultures. 3H-labeled collagen in microwell cultures was salt precipitated, harvested, and washed using a commercially available cell harvester and the filtered collagen was directly counted. The cell number could then be assessed either by methylene blue staining or by dissolving the cells in NaOH and estimating the protein content with bicinchoninic acid. In all of these procedures, the samples remain in microtiter plates, thus ensuring that minimal amounts of reagents are used and minimizing the amount of manipulation necessary. The intergroup variability is 3 to 9% and the recovery of 3H-labeled collagen is greater than 90%. Using this method, large numbers of samples can be assessed for collagen synthesis quickly, conveniently, and for minimal cost. KW - method AU - Scutt, A AU - Berg, A AU - Mayer, H PY - 1992/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/1384387 ER -

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