Immediate-early inducible function in upstream region of junB gene.

by: H Wan, H Ishihara, I Tanaka

Biomed Environ Sci, Vol. 19, No. 3. (June 2006), pp. 210-213.

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Abstract

OBJECTIVE: To analyze the upstream region of radiation-induced junB gene. METHODS: Four plasmids containing 250 bp, 590 bp, 900 bp and 1650 bp, and CAT reporter gene were constructed separately and introduced to L8704 cells. The cells were irradiated with 2 Gy X-rays and incubated at different intervals. Total RNA was extracted from the cells and fluctuation of the CAT mRNA level was assessed by the RNA ratio of CAT/beta-actin measured by quantitative Northern blot hybridization. RESULTS: CAT mRNA expression containing 900 bp and 1560 bp junB promoter remarkably and rapidly increased, and reached its peak 30 min after 2 Gy X-ray irradiation. CONCLUSIONS: 590-900 bp fragments located in the upstream region of junB gene play an important role in the early process of cells against radiation.

@article{citeulike:2289561, abstract = {OBJECTIVE: To analyze the upstream region of radiation-induced junB gene. METHODS: Four plasmids containing 250 bp, 590 bp, 900 bp and 1650 bp, and CAT reporter gene were constructed separately and introduced to L8704 cells. The cells were irradiated with 2 Gy X-rays and incubated at different intervals. Total RNA was extracted from the cells and fluctuation of the CAT mRNA level was assessed by the RNA ratio of CAT/beta-actin measured by quantitative Northern blot hybridization. RESULTS: CAT mRNA expression containing 900 bp and 1560 bp junB promoter remarkably and rapidly increased, and reached its peak 30 min after 2 Gy X-ray irradiation. CONCLUSIONS: 590-900 bp fragments located in the upstream region of junB gene play an important role in the early process of cells against radiation.}, address = {Beijing Neurosurgical Institute, China. wanhong502004@yahoo.com}, author = {Wan, H. and Ishihara, H. and Tanaka, I. }, citeulike-article-id = {2289561}, issn = {0895-3988}, journal = {Biomed Environ Sci}, month = {June}, number = {3}, pages = {210--213}, posted-at = {2008-01-25 14:57:27}, priority = {2}, title = {Immediate-early inducible function in upstream region of junB gene.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/16944778}, volume = {19}, year = {2006} }

RIS record

TY - JOUR ID - citeulike:2289561 L3 - citeulike-article-id:2289561 TI - Immediate-early inducible function in upstream region of junB gene. JF - Biomed Environ Sci VL - 19 IS - 3 SP - 210 EP - 213 AD - Beijing Neurosurgical Institute, China. wanhong502004@yahoo.com SN - 0895-3988 N2 - OBJECTIVE: To analyze the upstream region of radiation-induced junB gene. METHODS: Four plasmids containing 250 bp, 590 bp, 900 bp and 1650 bp, and CAT reporter gene were constructed separately and introduced to L8704 cells. The cells were irradiated with 2 Gy X-rays and incubated at different intervals. Total RNA was extracted from the cells and fluctuation of the CAT mRNA level was assessed by the RNA ratio of CAT/beta-actin measured by quantitative Northern blot hybridization. RESULTS: CAT mRNA expression containing 900 bp and 1560 bp junB promoter remarkably and rapidly increased, and reached its peak 30 min after 2 Gy X-ray irradiation. CONCLUSIONS: 590-900 bp fragments located in the upstream region of junB gene play an important role in the early process of cells against radiation. AU - Wan, H AU - Ishihara, H AU - Tanaka, I PY - 2006/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/16944778 ER -

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