Short RNA guides cleavage by eukaryotic RNase III.

@article{citeulike:1821296, abstract = {In eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution. However, the capacity of modern proteins to adopt new RNA guides has never been demonstrated. Here we show that Rnt1p, the yeast orthologue of the bacterial dsRNA-specific RNase III, can bind short RNA transcripts and use them as guides for sequence-specific cleavage. Target cleavage occurred at a constant distance from the Rnt1p binding site, leaving the guide RNA intact for subsequent cleavage. Our results indicate that RNase III may trigger sequence-specific RNA degradation independent of the RNAi machinery, and they open the road for a new generation of precise RNA silencing tools that do not trigger a dsRNA-mediated immune response.}, address = {Groupe ARN (RNA Group), Laboratoire de G\'{e}nomique Fonctionnelle (Laboratory for Functional Genomics), D\'{e}partement de Microbiologie et d'Infectiologie, Facult\'{e} de M\'{e}decine et des Sciences de la Sant\'{e}, Universit\'{e} de Sherbrooke, Sherbrooke, Qu\'{e}bec, Canada.}, author = {Lamontagne, B. and Abou Elela, S. }, citeulike-article-id = {1821296}, doi = {http://dx.doi.org/10.1371/journal.pone.0000472}, issn = {1932-6203}, journal = {PLoS ONE}, number = {5}, posted-at = {2007-10-25 16:14:15}, priority = {2}, title = {Short RNA guides cleavage by eukaryotic RNase III.}, url = {http://dx.doi.org/10.1371/journal.pone.0000472}, volume = {2}, year = {2007} }

TY - JOUR ID - citeulike:1821296 L3 - citeulike-article-id:1821296 TI - Short RNA guides cleavage by eukaryotic RNase III. JF - PLoS ONE VL - 2 IS - 5 AD - Groupe ARN (RNA Group), Laboratoire de Génomique Fonctionnelle (Laboratory for Functional Genomics), Département de Microbiologie et d'Infectiologie, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, Québec, Canada. SN - 1932-6203 N2 - In eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution. However, the capacity of modern proteins to adopt new RNA guides has never been demonstrated. Here we show that Rnt1p, the yeast orthologue of the bacterial dsRNA-specific RNase III, can bind short RNA transcripts and use them as guides for sequence-specific cleavage. Target cleavage occurred at a constant distance from the Rnt1p binding site, leaving the guide RNA intact for subsequent cleavage. Our results indicate that RNase III may trigger sequence-specific RNA degradation independent of the RNAi machinery, and they open the road for a new generation of precise RNA silencing tools that do not trigger a dsRNA-mediated immune response. AU - Lamontagne, B AU - Abou Elela, S PY - 2007/// UR - http://dx.doi.org/10.1371/journal.pone.0000472 ER -