The best control for the specificity of RNAi.

by: M Sarov, AF Stewart

Trends Biotechnol, Vol. 23, No. 9. (September 2005), pp. 446-448.

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Abstract

RNA interference (RNAi) is revolutionizing functional genomics. However, there are several reasons to be concerned about the specificity and off-target effects of this technique. A recent paper by Kittler et al. describes a straightforward way to validate RNAi specificity, which exploits the increasing availability of bacterial artificial chromosome (BAC) clone resources. Genetic rescue of the RNAi phenotype by BAC transgenesis is the best control yet described for specificity, and has further implications for reverse genetics.

@article{citeulike:1677967, abstract = {RNA interference (RNAi) is revolutionizing functional genomics. However, there are several reasons to be concerned about the specificity and off-target effects of this technique. A recent paper by Kittler et al. describes a straightforward way to validate RNAi specificity, which exploits the increasing availability of bacterial artificial chromosome (BAC) clone resources. Genetic rescue of the RNAi phenotype by BAC transgenesis is the best control yet described for specificity, and has further implications for reverse genetics.}, address = {Genomics, BioInnovationZentrum, The University of Technology, Dresden, Am Tatzberg 47, 01307 Dresden, Germany.}, author = {Sarov, M. and Stewart, A. F. }, citeulike-article-id = {1677967}, doi = {10.1016/j.tibtech.2005.06.007}, issn = {0167-7799}, journal = {Trends Biotechnol}, keywords = {rnai}, month = {September}, number = {9}, pages = {446--448}, posted-at = {2007-09-20 04:22:26}, priority = {2}, title = {The best control for the specificity of RNAi.}, url = {http://dx.doi.org/10.1016/j.tibtech.2005.06.007}, volume = {23}, year = {2005} }

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TY - JOUR ID - citeulike:1677967 L3 - citeulike-article-id:1677967 TI - The best control for the specificity of RNAi. JF - Trends Biotechnol VL - 23 IS - 9 SP - 446 EP - 448 AD - Genomics, BioInnovationZentrum, The University of Technology, Dresden, Am Tatzberg 47, 01307 Dresden, Germany. SN - 0167-7799 N2 - RNA interference (RNAi) is revolutionizing functional genomics. However, there are several reasons to be concerned about the specificity and off-target effects of this technique. A recent paper by Kittler et al. describes a straightforward way to validate RNAi specificity, which exploits the increasing availability of bacterial artificial chromosome (BAC) clone resources. Genetic rescue of the RNAi phenotype by BAC transgenesis is the best control yet described for specificity, and has further implications for reverse genetics. KW - rnai AU - Sarov, M AU - Stewart, AF PY - 2005/09// UR - http://dx.doi.org/10.1016/j.tibtech.2005.06.007 ER -

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