Multi-dimensional HPLC/MS of the nucleolar proteome using HPLC-chip/MS

@article{citeulike:1509912, abstract = {The proteome of the human nucleolus was investigated in a single analysis using off-line strong cation exchange chromatography and microfraction collection combined with HPLC-chip/MS. The analysis was conducted either as a 1-D workflow with HPLC-chip alone or as a 2-D workflow. Two hundred and six unique proteins were identified in the International Protein Index human database corresponding to 2024 unique tryptic peptides identified in the 2-D analysis. In contrast, only 34 proteins and 151 corresponding tryptic peptides were found by applying a 1-D separation strategy. This clearly indicated that the complexity of the samples required the combination of more than one orthogonal separation technique. Stringent database search criteria, including reversal of sequences and therefore better exclusion of false-positive identifications, were applied for reliable protein identification.}, address = {Agilent Technologies, Waldbronn, Germany. Fax: +49-0-7243-602414; Biomedical Proteomics Research Group, Geneva University Hospital and Medical Center, Geneva, Switzerland; Agilent Technologies, Santa Clara, CA, USA; Faculty of Sciences Geneva University, Pharmacy Section, Geneva, Switzerland}, author = {Vollmer, Martin and H\"{o}rth, Patric and Rozing, Gerard and Cout\'{e}, Yohann and Grimm, Rudi and Hochstrasser, Denis and Sanchez, Jean-Charles }, citeulike-article-id = {1509912}, doi = {10.1002/jssc.200500334}, journal = {Journal of Separation Science}, number = {4}, pages = {499--509}, posted-at = {2007-07-28 11:18:36}, priority = {2}, title = {Multi-dimensional HPLC/MS of the nucleolar proteome using HPLC-chip/MS}, url = {http://dx.doi.org/10.1002/jssc.200500334}, volume = {29}, year = {2006} }

TY - JOUR ID - citeulike:1509912 L3 - citeulike-article-id:1509912 TI - Multi-dimensional HPLC/MS of the nucleolar proteome using HPLC-chip/MS JF - Journal of Separation Science VL - 29 IS - 4 SP - 499 EP - 509 AD - Agilent Technologies, Waldbronn, Germany. Fax: +49-0-7243-602414; Biomedical Proteomics Research Group, Geneva University Hospital and Medical Center, Geneva, Switzerland; Agilent Technologies, Santa Clara, CA, USA; Faculty of Sciences Geneva University, Pharmacy Section, Geneva, Switzerland N2 - The proteome of the human nucleolus was investigated in a single analysis using off-line strong cation exchange chromatography and microfraction collection combined with HPLC-chip/MS. The analysis was conducted either as a 1-D workflow with HPLC-chip alone or as a 2-D workflow. Two hundred and six unique proteins were identified in the International Protein Index human database corresponding to 2024 unique tryptic peptides identified in the 2-D analysis. In contrast, only 34 proteins and 151 corresponding tryptic peptides were found by applying a 1-D separation strategy. This clearly indicated that the complexity of the samples required the combination of more than one orthogonal separation technique. Stringent database search criteria, including reversal of sequences and therefore better exclusion of false-positive identifications, were applied for reliable protein identification. AU - Vollmer, Martin AU - Hörth, Patric AU - Rozing, Gerard AU - Couté, Yohann AU - Grimm, Rudi AU - Hochstrasser, Denis AU - Sanchez, Jean-Charles PY - 2006/// UR - http://dx.doi.org/10.1002/jssc.200500334 ER -