Direct analysis and MALDI imaging of formalin-fixed, paraffin-embedded tissue sections.

@article{citeulike:1462861, abstract = {Formalin fixation, generally followed by paraffin embedding, is the standard and well-established processing method employed by pathologist. This treatment conserves and stabilizes biopsy samples for years. Analysis of FFPE tissues from biopsy libraries has been, so far, a challenge for proteomics biomarker studies. Herein, we present two methods for the direct analysis of formalin-fixed, paraffin-embedded (FFPE) tissues by MALDI-MS. The first is based on the use of a reactive matrix, 2,4-dinitrophenylhydrazine, useful for FFPE tissues stored less than 1 year. The second approach is applicable for all FFPE tissues regardless of conservation time. The strategy is based on in situ enzymatic digestion of the tissue section after paraffin removal. In situ digestion can be performed on a specific area of the tissue as well as on a very small area (microdigestion). Combining automated microdigestion of a predefined tissue array with either in situ extraction prior to classical nanoLC/MS-MS analysis or automated microspotting of MALDI matrix according to the same array allows the identification of both proteins by nanoLC-nanoESI and MALDI imaging. When adjacent tissue sections are used, it is, thus, possible to correlate protein identification and molecular imaging. These combined approaches, along with FFPE tissue analysis provide access to massive amounts of archived samples in the clinical pathology setting.}, address = {Laboratoire de Neuroimmunologie des Ann\'{e}lides, FRE-CNRS 2933, MALDI Imaging Team, Cit\'{e} Scientifique, Universit\'{e} des Sciences et Technologies de Lille, 59650 Villeneuve d'Ascq, France.}, author = {Lemaire, R. and Desmons, A. and Tabet, J. C. and Day, R. and Salzet, M. and Fournier, I. }, citeulike-article-id = {1462861}, doi = {10.1021/pr060549i}, issn = {1535-3893}, journal = {J Proteome Res}, keywords = {ffpe}, month = {April}, number = {4}, pages = {1295--1305}, posted-at = {2007-07-17 16:45:29}, priority = {2}, title = {Direct analysis and MALDI imaging of formalin-fixed, paraffin-embedded tissue sections.}, url = {http://dx.doi.org/10.1021/pr060549i}, volume = {6}, year = {2007} }

TY - JOUR ID - citeulike:1462861 L3 - citeulike-article-id:1462861 TI - Direct analysis and MALDI imaging of formalin-fixed, paraffin-embedded tissue sections. JF - J Proteome Res VL - 6 IS - 4 SP - 1295 EP - 1305 AD - Laboratoire de Neuroimmunologie des Annélides, FRE-CNRS 2933, MALDI Imaging Team, Cité Scientifique, Université des Sciences et Technologies de Lille, 59650 Villeneuve d'Ascq, France. SN - 1535-3893 N2 - Formalin fixation, generally followed by paraffin embedding, is the standard and well-established processing method employed by pathologist. This treatment conserves and stabilizes biopsy samples for years. Analysis of FFPE tissues from biopsy libraries has been, so far, a challenge for proteomics biomarker studies. Herein, we present two methods for the direct analysis of formalin-fixed, paraffin-embedded (FFPE) tissues by MALDI-MS. The first is based on the use of a reactive matrix, 2,4-dinitrophenylhydrazine, useful for FFPE tissues stored less than 1 year. The second approach is applicable for all FFPE tissues regardless of conservation time. The strategy is based on in situ enzymatic digestion of the tissue section after paraffin removal. In situ digestion can be performed on a specific area of the tissue as well as on a very small area (microdigestion). Combining automated microdigestion of a predefined tissue array with either in situ extraction prior to classical nanoLC/MS-MS analysis or automated microspotting of MALDI matrix according to the same array allows the identification of both proteins by nanoLC-nanoESI and MALDI imaging. When adjacent tissue sections are used, it is, thus, possible to correlate protein identification and molecular imaging. These combined approaches, along with FFPE tissue analysis provide access to massive amounts of archived samples in the clinical pathology setting. KW - ffpe AU - Lemaire, R AU - Desmons, A AU - Tabet, JC AU - Day, R AU - Salzet, M AU - Fournier, I PY - 2007/04// UR - http://dx.doi.org/10.1021/pr060549i ER -