Analysis of cell cycle phases and progression in cultured mammalian cells

by: Christoph Schorl, John M Sedivy

Methods, Vol. 41, No. 2. (February 2007), pp. 143-150.

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Abstract

Fluorescence activated cell sorting (FACS) analysis has become a standard tool to analyze cell cycle distributions in populations of cells. These methods require relatively large numbers of cells, and do not provide optimal resolution of the transitions between cell cycle phases. In this report we describe in detail complementary methods that utilize the incorporation of nucleotide analogs combined with microscopic examination. While often more time consuming, these protocols typically require far fewer cells, and allow accurate kinetic assessment of cell cycle progression. We also describe the use of a technique for the synchronization of adherent cells in mitosis by simple mechanical agitation (mitotic shake-off) that eliminates physiological perturbation associated with drug treatments.

@article{citeulike:1447714, abstract = {Fluorescence activated cell sorting (FACS) analysis has become a standard tool to analyze cell cycle distributions in populations of cells. These methods require relatively large numbers of cells, and do not provide optimal resolution of the transitions between cell cycle phases. In this report we describe in detail complementary methods that utilize the incorporation of nucleotide analogs combined with microscopic examination. While often more time consuming, these protocols typically require far fewer cells, and allow accurate kinetic assessment of cell cycle progression. We also describe the use of a technique for the synchronization of adherent cells in mitosis by simple mechanical agitation (mitotic shake-off) that eliminates physiological perturbation associated with drug treatments.}, author = {Schorl, Christoph and Sedivy, John M. }, booktitle = {Methods in Cell Cycle Research}, citeulike-article-id = {1447714}, journal = {Methods}, month = {February}, number = {2}, pages = {143--150}, posted-at = {2007-07-11 05:56:12}, priority = {2}, title = {Analysis of cell cycle phases and progression in cultured mammalian cells}, url = {http://www.sciencedirect.com/science/article/B6WN5-4MMWF6T-3/2/1ba613c31cb6b00fc399a26a01dc25d4}, volume = {41}, year = {2007} }

RIS record

TY - JOUR ID - citeulike:1447714 L3 - citeulike-article-id:1447714 TI - Analysis of cell cycle phases and progression in cultured mammalian cells T2 - Methods in Cell Cycle Research JF - Methods VL - 41 IS - 2 SP - 143 EP - 150 N2 - Fluorescence activated cell sorting (FACS) analysis has become a standard tool to analyze cell cycle distributions in populations of cells. These methods require relatively large numbers of cells, and do not provide optimal resolution of the transitions between cell cycle phases. In this report we describe in detail complementary methods that utilize the incorporation of nucleotide analogs combined with microscopic examination. While often more time consuming, these protocols typically require far fewer cells, and allow accurate kinetic assessment of cell cycle progression. We also describe the use of a technique for the synchronization of adherent cells in mitosis by simple mechanical agitation (mitotic shake-off) that eliminates physiological perturbation associated with drug treatments. AU - Schorl, Christoph AU - Sedivy, John PY - 2007/02// UR - http://www.sciencedirect.com/science/article/B6WN5-4MMWF6T-3/2/1ba613c31cb6b00fc399a26a01dc25d4 ER -

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