Epidermal growth factor receptor-induced activator protein 1 activity controls density-dependent growth inhibition in normal rat kidney fibroblasts.

@article{citeulike:1369361, abstract = {Density-dependent growth inhibition secures tissue homeostasis. Dysfunction of the mechanisms, which regulate this type of growth control is a major cause of neoplasia. In confluent normal rat kidney (NRK) fibroblasts, epidermal growth factor (EGF) receptor levels decline, ultimately rendering these cells irresponsive to EGF. Using an activator protein (AP)-1 sensitive reporter construct, we show that AP-1 activity is strongly decreased in density-arrested NRK cells, but is restored after relaxation of densitydependent growth inhibition by removing neighboring cells. EGF could not induce AP-1 activity or S-phase entry in density-arrested cells, but could do so after pretreatment with retinoic acid, which enhances EGF receptor expression. Our results support a model in which the EGF receptor regulates density-dependent growth control in NRK fibroblasts, which is reflected by EGF-induced mitogenic signaling and consequent AP-1 activity.}, address = {Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, Vrije Universiteit, Amsterdam, the Netherlands. jorrit.hornberg@organon.com}, author = {Hornberg, J. J. and Dekker, H. and Peters, P. H. and Langerak, P. and Westerhoff, H. V. and Lankelma, J. and van Zoelen, E. J. }, citeulike-article-id = {1369361}, issn = {1073-6085}, journal = {Mol Biotechnol}, month = {October}, number = {2}, pages = {101--108}, posted-at = {2007-06-07 05:50:50}, priority = {2}, title = {Epidermal growth factor receptor-induced activator protein 1 activity controls density-dependent growth inhibition in normal rat kidney fibroblasts.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17172655}, volume = {34}, year = {2006} }

TY - JOUR ID - citeulike:1369361 L3 - citeulike-article-id:1369361 TI - Epidermal growth factor receptor-induced activator protein 1 activity controls density-dependent growth inhibition in normal rat kidney fibroblasts. JF - Mol Biotechnol VL - 34 IS - 2 SP - 101 EP - 108 AD - Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, Vrije Universiteit, Amsterdam, the Netherlands. jorrit.hornberg@organon.com SN - 1073-6085 N2 - Density-dependent growth inhibition secures tissue homeostasis. Dysfunction of the mechanisms, which regulate this type of growth control is a major cause of neoplasia. In confluent normal rat kidney (NRK) fibroblasts, epidermal growth factor (EGF) receptor levels decline, ultimately rendering these cells irresponsive to EGF. Using an activator protein (AP)-1 sensitive reporter construct, we show that AP-1 activity is strongly decreased in density-arrested NRK cells, but is restored after relaxation of densitydependent growth inhibition by removing neighboring cells. EGF could not induce AP-1 activity or S-phase entry in density-arrested cells, but could do so after pretreatment with retinoic acid, which enhances EGF receptor expression. Our results support a model in which the EGF receptor regulates density-dependent growth control in NRK fibroblasts, which is reflected by EGF-induced mitogenic signaling and consequent AP-1 activity. AU - Hornberg, JJ AU - Dekker, H AU - Peters, PH AU - Langerak, P AU - Westerhoff, HV AU - Lankelma, J AU - van Zoelen, EJ PY - 2006/10// UR - http://view.ncbi.nlm.nih.gov/pubmed/17172655 ER -