A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays.

@article{citeulike:482842, abstract = {The ability to identify active compounds (³hits²) from large chemical libraries accurately and rapidly has been the ultimate goal in developing high-throughput screening (HTS) assays. The ability to identify hits from a particular HTS assay depends largely on the suitability or quality of the assay used in the screening. The criteria or parameters for evaluating the ³suitability² of an HTS assay for hit identification are not well defined and hence it still remains difficult to compare the quality of assays directly. In this report, a screening window coefficient, called ³Z-factor,² is defined. This coefficient is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment. The Z-factor is a dimensionless, simple statistical characteristic for each HTS assay. The Z-factor provides a useful tool for comparison and evaluation of the quality of assays, and can be utilized in assay optimization and validation.}, address = {DuPont Pharmaceuticals Research Laboratories, Leads Discovery, DuPont Pharmaceuticals Company, Wilmington, Delaware.}, author = {Zhang, J. H. and Chung, T. D. and Oldenburg, K. R. }, citeulike-article-id = {482842}, doi = {10.1177/108705719900400206}, issn = {1087-0571}, journal = {J Biomol Screen}, keywords = {cytotoxicity}, number = {2}, pages = {67--73}, posted-at = {2008-02-22 16:55:47}, priority = {2}, title = {A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays.}, url = {http://dx.doi.org/10.1177/108705719900400206}, volume = {4}, year = {1999} }

TY - JOUR ID - citeulike:482842 L3 - citeulike-article-id:482842 TI - A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. JF - J Biomol Screen VL - 4 IS - 2 SP - 67 EP - 73 AD - DuPont Pharmaceuticals Research Laboratories, Leads Discovery, DuPont Pharmaceuticals Company, Wilmington, Delaware. SN - 1087-0571 N2 - The ability to identify active compounds (³hits²) from large chemical libraries accurately and rapidly has been the ultimate goal in developing high-throughput screening (HTS) assays. The ability to identify hits from a particular HTS assay depends largely on the suitability or quality of the assay used in the screening. The criteria or parameters for evaluating the ³suitability² of an HTS assay for hit identification are not well defined and hence it still remains difficult to compare the quality of assays directly. In this report, a screening window coefficient, called ³Z-factor,² is defined. This coefficient is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment. The Z-factor is a dimensionless, simple statistical characteristic for each HTS assay. The Z-factor provides a useful tool for comparison and evaluation of the quality of assays, and can be utilized in assay optimization and validation. KW - cytotoxicity AU - Zhang, JH AU - Chung, TD AU - Oldenburg, KR PY - 1999/// UR - http://dx.doi.org/10.1177/108705719900400206 ER -