Structures of ruthenium-modified Pseudomonas aeruginosa azurin and [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O.

@article{citeulike:1790220, abstract = {The crystal structure of Ru(2, 2'-bipyridine)2(imidazole)(His83)azurin (RuAz) has been determined to 2.3 A -resolution by X-ray crystallography. The spectroscopic and thermodynamic properties of both the native protein and [Ru(2, 2'-bipyridine)2(imidazole)2]2+ are maintained in the modified protein. Dark-green RuAz crystals grown from PEG 4000, LiNO3, CuCl2 and Tris buffer are monoclinic, belong to the space group C2 and have cell parameters a = 100.6, b = 35.4, c = 74.7 A and beta = 106. 5 degrees. In addition, [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O was synthesized, crystallized and structurally characterized by X-ray crystallography. Red-brown crystals of this complex are monoclinic, space group P21/n, unit-cell parameters a = 13.230 (2), b = 18.197 (4), c = 16.126 (4) A, beta = 108.65 (2) degrees. Stereochemical parameters for the refinement of Ru(2, 2'-bipyridine)2(imidazole)(His83) were taken from the atomic coordinates of [Ru(2,2'-bipyridine)2(imidazole)2]2+. The structure of RuAz confirms that His83 is the only site of chemical modification and that the native azurin structure is not perturbed significantly by the ruthenium label.}, address = {Division of Chemistry and Chemical Engineering, 147-75 CH, California Institute of Technology, Pasadena, California 91125, USA.}, author = {Faham, S. and Day, M. W. and Connick, W. B. and Crane, B. R. and Di Bilio, A. J. and Schaefer, W. P. and Rees, D. C. and Gray, H. B. }, citeulike-article-id = {1790220}, issn = {0907-4449}, journal = {Acta Crystallogr D Biol Crystallogr}, keywords = {artificial\_photosynthesis, protein\_design}, month = {February}, number = {Pt 2}, pages = {379--385}, posted-at = {2007-10-19 20:08:24}, priority = {2}, title = {Structures of ruthenium-modified Pseudomonas aeruginosa azurin and [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10089343}, volume = {55}, year = {1999} }

TY - JOUR ID - citeulike:1790220 L3 - citeulike-article-id:1790220 TI - Structures of ruthenium-modified Pseudomonas aeruginosa azurin and [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O. JF - Acta Crystallogr D Biol Crystallogr VL - 55 IS - Pt 2 SP - 379 EP - 385 AD - Division of Chemistry and Chemical Engineering, 147-75 CH, California Institute of Technology, Pasadena, California 91125, USA. SN - 0907-4449 N2 - The crystal structure of Ru(2, 2'-bipyridine)2(imidazole)(His83)azurin (RuAz) has been determined to 2.3 A -resolution by X-ray crystallography. The spectroscopic and thermodynamic properties of both the native protein and [Ru(2, 2'-bipyridine)2(imidazole)2]2+ are maintained in the modified protein. Dark-green RuAz crystals grown from PEG 4000, LiNO3, CuCl2 and Tris buffer are monoclinic, belong to the space group C2 and have cell parameters a = 100.6, b = 35.4, c = 74.7 A and beta = 106. 5 degrees. In addition, [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O was synthesized, crystallized and structurally characterized by X-ray crystallography. Red-brown crystals of this complex are monoclinic, space group P21/n, unit-cell parameters a = 13.230 (2), b = 18.197 (4), c = 16.126 (4) A, beta = 108.65 (2) degrees. Stereochemical parameters for the refinement of Ru(2, 2'-bipyridine)2(imidazole)(His83) were taken from the atomic coordinates of [Ru(2,2'-bipyridine)2(imidazole)2]2+. The structure of RuAz confirms that His83 is the only site of chemical modification and that the native azurin structure is not perturbed significantly by the ruthenium label. KW - artificial_photosynthesis KW - protein_design AU - Faham, S AU - Day, MW AU - Connick, WB AU - Crane, BR AU - Di Bilio, AJ AU - Schaefer, WP AU - Rees, DC AU - Gray, HB PY - 1999/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/10089343 ER -