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jonathanjacobs library [55 articles]

最近 jonathanjacobs さんのライブラリ .
  • RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts
    Nat. Protocols, Vol. 1, No. 1. (June 2006), pp. 302-307.
    by Jack D Keene, Jordan M Komisarow, Matthew B Friedersdorf
    posted to affinity genomics rna rnp by jonathanjacobs on 2008-08-29 00:49:33 as **
  • Combining SELEX and the yeast three-hybrid system for in vivo selection and classification of RNA aptamers.
    RNA, Vol. 13, No. 4. (April 2007), pp. 614-622.
    by J König, C Julius, S Baumann, M Homann, HU Göringer, M Feldbrügge
    posted to hybrid rna selection selex three yeast by jonathanjacobs on 2008-08-27 22:50:19 as read along with 2 people jyuh yeastyboy
  • The La antigen shuttles between the nucleus and the cytoplasm in CV-1 cells.
    Molecular and cellular biochemistry, Vol. 85, No. 2. (21 February 1989), pp. 103-114.
    by M Bachmann, K Pfeifer, HC Schröder, WE Müller
    posted to cytoplasm la nucleus shuttling by jonathanjacobs on 2008-08-26 02:30:04 as **
  • RNA sequence and secondary structure participate in high-affinity CsrA-RNA interaction
    RNA, Vol. 11, No. 10. (October 2005), pp. 1579-1587.
    by AK Dubey, CS Baker, T Romeo, P Babitzke
    posted to protocol rna selex structure by jonathanjacobs on 2008-08-26 02:23:23 as read
  • Differential Phosphorylation and Subcellular Localization of La RNPs Associated with Precursor tRNAs and Translation-Related mRNAs
    Molecular Cell, Vol. 12, No. 5. (November 2003), pp. 1301-1307.
    by Robert V Intine, Scott A Tenenbaum, Amy L Sakulich, Jack D Keene, Richard J Maraia
    posted to la localization phosphoprotein rnp translation by jonathanjacobs on 2008-08-26 02:15:47 as read
  • The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p
    Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 10. (May 2000), pp. 5273-5278.
    by Peter A Takizawa, Ronald D Vale
    posted to ash1 localization mrna myo4 protocol rna she3 by jonathanjacobs on 2008-08-26 02:12:32 as read
  • Two Yeast La Motif-containing Proteins Are RNA-binding Proteins that Associate with Polyribosomes
    Mol. Biol. Cell, Vol. 10, No. 11. (1 November 1999), pp. 3849-3862.
    by Suzanne G Sobel, Sandra L Wolin
    posted to la lhp1 ribosome rna slf1p sro9p translation by jonathanjacobs on 2008-08-26 02:10:28 as read
  • Libraries for genomic SELEX
    Nucl. Acids Res., Vol. 25, No. 4. (15 February 1997), pp. 781-786.
    by BS Singer, T Shtatland, D Brown, L Gold
    posted to rna selex by jonathanjacobs on 2008-08-26 01:57:47 as read along with 1 group Molecular Biology
  • Trading translation with RNA-binding proteins
    RNA, Vol. 14, No. 3. (March 2008), pp. 404-409.
    by I Abaza, F Gebauer
    posted to mrnas protein regulation ribonucleoprotein rna rna-binding translation by jonathanjacobs on 2008-08-25 23:36:30 as read
  • Ribonucleoprotein infrastructure regulating the flow of genetic information between the genome and the proteome
    Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 13. (19 June 2001), pp. 7018-7024.
    by Jack D Keene
    posted to gene mrna network operons polyabinding polycistronic protein regulons ribonucleoproteins rna rnabinding translation by jonathanjacobs on 2008-08-25 23:31:13 as ** along with 1 person klo
  • RNA regulons: coordination of post-transcriptional events
    Nature Reviews Genetics, Vol. 8, No. 7., pp. 533-543.
    by Jack D Keene
    posted to expression gene mrnas protein regulons rna translation by jonathanjacobs on 2008-08-25 23:27:03 as read along with 2 people and 2 groups jyuh ajgentles Molecular Biology PROTEIN TRANSLATION
  • Size, constant sequences, and optimal selection
    RNA, Vol. 11, No. 11. (1 November 2005), pp. 1701-1709.
    by Michal Legiewicz, Catherine Lozupone, ROB Knight, Michael Yarus
    posted to aptamer dna evolution rna selex structure by jonathanjacobs on 2008-07-17 21:50:32 as ***** along with 1 group Molecular Biology
  • PRFdb: A database of computationally predicted eukaryotic programmed -1 ribosomal frameshift signals
    BMC Genomics, Vol. 9, No. 1. (2008)
    by Ashton Belew, Nicholaus Hepler, Jonathan Jacobs, Jonathan Dinman
    posted to bioinformatics biology cerevisiae computational database decay frameshift genome mrna nmd pseudoknot rna saccharomyces structure translation virus yeast by jonathanjacobs on 2008-07-17 17:12:43 as read
  • Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution
    Nature (18 May 2008)
    by Brian T Wilhelm, Samuel Marguerat, Stephen Watt, Falk Schubert, Valerie Wood, Ian Goodhead, Christopher J Penkett, Jane Rogers, Jürg Bähler
    posted to array cell dna exon gene genome intron pombe rna schizosaccharomyces sequence transcription transcriptome yeast by jonathanjacobs on 2008-06-26 12:17:44 as ** along with 6 people and 1 group caseybrown myourshaw djkt GeeSharpMinor jyuh semrich Molecular Biology
  • Diversity of Oligonucleotide Functions
    Annual Review of Biochemistry, Vol. 64, No. 1. (1995), pp. 763-797.
    by L Gold, B Polisky, O Uhlenbeck, M Yarus
    posted to dna ligands oligonucleotide protein rna selex structure by jonathanjacobs on 2008-06-23 16:20:18 as **
  • THE LA PROTEIN
    Annual Review of Biochemistry, Vol. 71, No. 1. (2002), pp. 375-403.
    by Sandra L Wolin, Tommy Cedervall
    posted to cell data eukaryotic genetic la mrnas protein review rna translation trna by jonathanjacobs on 2008-06-20 18:21:04 as read along with 1 group The La Autoantigen.
  • In vitro selection of RNA molecules that bind specific ligands
    Nature, Vol. 346, No. 6287. (1990), pp. 818-822.
    by Andrew D Ellington, Jack W Szostak
    posted to evolution ligand rna selex sequence by jonathanjacobs on 2008-06-16 15:20:06 as read
  • Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.
    Science, Vol. 249, No. 4968. (3 August 1990), pp. 505-510.
    by C Tuerk, L Gold
    posted to affinity dna evolution ligand mrna protein rna selex sequence by jonathanjacobs on 2008-06-16 15:17:45 as read along with 2 people and 2 groups indigoviolet leelarcombe EisenLab Molecular Biology
  • RNA sequence and secondary structure participate in high-affinity CsrA-RNA interaction
    RNA, Vol. 11, No. 10. (1 October 2005), pp. 1579-1587.
    by Ashok K Dubey, Carol S Baker, TONY Romeo, PAUL Babitzke
    posted to affinity consensus evolution gene ligand mrna nucleotide protein ribosome rna selex structure translation by jonathanjacobs on 2008-06-16 15:15:30 as read
  • Identification of RNA ligands that bind hepatitis C virus polymerase selectively and inhibit its RNA synthesis from the natural viral RNA templates
    Virology, Vol. 307, No. 2. (15 March 2003), pp. 301-316.
    by Nam V Vo, Jong-Won Oh, Michael M Lai
    posted to aptamer conserved evolution hcv ligand nucleotide polymerase protein purine pyrimidine rna selex sequence structure viral by jonathanjacobs on 2008-06-16 15:11:45 as **
  • SELEX--a (r)evolutionary method to generate high-affinity nucleic acid ligands.
    Biomol Eng, Vol. 24, No. 4. (October 2007), pp. 381-403.
    by R Stoltenburg, C Reinemann, B Strehlitz
    posted to affinity aptamer dna evolution ligands molecular oligonucleotide polymerase protein proteomics research rna science selection selex by jonathanjacobs on 2008-05-14 18:48:28 as read along with 1 person jyuh
  • Genomic systematic evolution of ligands by exponential enrichment (Genomic SELEX) for the identification of protein-binding RNAs independent of their expression levels
    Nat. Protocols, Vol. 1, No. 5. (December 2006), pp. 2204-2212.
    by Christina Lorenz, Frederike von Pelchrzim, Renee Schroeder
    posted to affinity dna evolution experimental expression gene genomics pcr protein protocol rna selex transcription by jonathanjacobs on 2008-05-14 01:36:45 as read along with 1 group Molecular Biology
  • High-Throughput SHAPE Analysis Reveals Structures in HIV-1 Genomic RNA Strongly Conserved across Distinct Biological States
    PLoS Biology, Vol. 6, No. 4. (1 April 2008), e96.
    by Kevin A Wilkinson, Robert J Gorelick, Suzy M Vasa, Nicolas Guex, Alan Rein, David H Mathews, Morgan C Giddings, Kevin M Weeks
    posted to dna fold function genome genomics hiv homolog human motif nascent native noncoding nucleocapsid nucleotide primer protein retroviral ribosome rna rnaprotein sequence shape structure transcriptase transcriptome trna virus by jonathanjacobs on 2008-05-08 16:40:11 as ***** along with 1 person and 1 group kieran101 Molecular Biology
  • Posttranscriptional Gene Regulation by Spatial Rearrangement of the 3′ Untranslated Region
    PLoS Biology, Vol. 6, No. 4. (1 April 2008), e92.
    by Andrea B Eberle, Lukas Stalder, Hansruedi Mathys, Rodolfo Z Orozco, Oliver Mühlemann
    posted to cell codon conserved decay degradation evolution folding function gene human mechanism mrna nmd nonsensemediated pabpc pathway polya polyabinding posttranscriptional protein ptc regulation termination translation untranslated by jonathanjacobs on 2008-04-30 15:38:52 as *** along with 1 group PROTEIN TRANSLATION
  • Identification of novel posttranscriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis
    Blood, Vol. 111, No. 2. (15 January 2008), pp. 816-828.
    by Anna M Eiring, Paolo Neviani, Ramasamy Santhanam, Joshua J Oaks, Ji S Chang, Mario Notari, William Willis, Carlo Gambacorti-Passerini, Stefano Volinia, Guido Marcucci, Michael A Caligiuri, Gustavo W Leone, Danilo Perrotti
    posted to apoptotic cell conserved development differentiation expression function hnrnpa kinase knockdown leukemia marrow mice mrna myelogenous myeloid network oncoprotein posttranscriptional posttranslational protein rbps regulation rna shrna stimuli survival transcription untranslated upregulated utr vivo by jonathanjacobs on 2008-04-18 22:55:15 as **** along with 1 group The La Autoantigen.
  • The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression
    FEBS Journal, Vol. 272, No. 9. (2005), pp. 2118-2131.
    by Christophe Maris, Cyril Dominguez, Frederic HT Allain
    posted to conserved eukaryotes function motif protein review ribonucleoprotein rna rnp rrm sequences structure by jonathanjacobs on 2008-04-10 16:51:30 as ***** along with 1 person and 2 groups marck Molecular Biology The La Autoantigen.
  • Downregulation of the essential Trypanosoma brucei La protein affects accumulation of elongator methionyl-tRNA
    Molecular and Biochemical Parasitology, Vol. 144, No. 1. (November 2005), pp. 104-108.
    by George K Arhin, Shuiyuan Shen, Iehsus F Perez, Christian Tschudi, Elisabetta Ullu
    posted to cerevisiae chaperone conserved cytoplasmic database degradation endonucleolytic eukaryotes eukaryotic evolution experimental function gene homologue human la methionylpretrna microarray molecular motif mrnas nuclear nuclease nucleolar oligou phosphoprotein pol polymerase polyuridine prerrna presl pretrnas protein protozoa ribonucleoprotein rna saccharomyces snorna snrna splicing transcription trna trypanosome by jonathanjacobs on 2008-04-08 19:59:38 as **
  • The Trypanosoma brucei La protein is a candidate poly(U) shield that impacts spliced leader RNA maturation and tRNA intron removal
    International Journal for Parasitology, Vol. 35, No. 4. (1 April 2005), pp. 359-366.
    by Silvie Foldynova-Trantirkova, Zdenek Paris, Nancy R Sturm, David A Campbell, Julius Lukes
    posted to eukaryotic gene intron la lethality methylation motif mrna nuclear nucleolar pathway polyu protein ribosome rna splicing transsplicing trnatyr trypanosoma vector viable yeast by jonathanjacobs on 2008-04-08 19:55:29 as ** along with 1 group The La Autoantigen.
  • Genetic analysis of a La homolog in Drosophila melanogaster
    Nucl. Acids Res., Vol. 28, No. 5. (1 March 2000), pp. 1078-1084.
    by Chunyang Bai, Peter P Tolias
    posted to conserved development drosophila embryo embryonic eukaryote gene genetic homolog hypothesis ires la larvae melanogaster morphogenesis polymerase protein ribosome rna translational vivo by jonathanjacobs on 2008-04-08 19:52:25 as ** along with 1 group The La Autoantigen.
  • A bona fide La protein is required for embryogenesis in Arabidopsis thaliana
    Nucl. Acids Res., Vol. 35, No. 10. (11 May 2007), pp. 3306-3321.
    by Sophie Fleurdepine, Jean-Marc Deragon, Martine Devic, Jocelyne Guilleminot, Cecile Bousquet-Antonelli
    posted to arabidopsis biogenesis cerevisiae development embryogenesis embryonic genome la lethal motif mutant nuclear nucleolar nucleoplasm phenotype phylogenetic plant protein ribosome rna saccharomyces thaliana by jonathanjacobs on 2008-04-08 19:44:53 as read along with 1 group The La Autoantigen.
  • Following translation by single ribosomes one codon at a time
    Nature (09 March 2008)
    by Jin-Der Wen, Laura Lancaster, Courtney Hodges, Ana-Carolina Zeri, Shige H Yoshimura, Harry F Noller, Carlos Bustamante, Ignacio Tinoco
    posted to codon hairpins mrna ribosome rna structure translation translocation tweezers by jonathanjacobs on 2008-04-02 22:44:09 as read along with 4 people dchughes melting simone cactus
  • Cap-Independent Translation Is Required for Starvation-Induced Differentiation in Yeast
    Science, Vol. 317, No. 5842. (31 August 2007), pp. 1224-1227.
    by Wendy V Gilbert, Kaihong Zhou, Tamira K Butler, Jennifer A Doudna
    posted to cellular developmental genes growth initiation ires mechanism molecular mrna mutation pab pathway polya protein ribosome rna stress structure synthesis translation untranslated utr viral yeast by jonathanjacobs on 2008-04-02 16:23:19 as read along with 1 person and 1 group balabu Molecular Biology
  • The multifunctional RNA-binding protein La is required for mouse development and for the establishment of embryonic stem cells.
    Mol Cell Biol, Vol. 26, No. 4. (February 2006), pp. 1445-1451.
    by JM Park, MJ Kohn, MW Bruinsma, C Vech, RV Intine, S Fuhrmann, A Grinberg, I Mukherjee, PE Love, MS Ko, ML DePamphilis, RJ Maraia
    posted to biogenesis cell cells cerevisiae development embryonic erythematosus eukaryote gene la lupus mice mrna noncoding polymerase pombe protein ribosome rna saccharomyces schizosaccharomyces sjogrens snornas trna yeast by jonathanjacobs on 2008-03-31 21:28:15 as *** along with 1 person and 1 group miguel The La Autoantigen.
  • A La-Related Protein Modulates 7SK snRNP Integrity to Suppress P-TEFb-Dependent Transcriptional Elongation and Tumorigenesis
    Molecular Cell, Vol. 29, No. 5. (14 March 2008), pp. 588-599.
    by Nanhai He, Nadine S Jahchan, Eunmee Hong, Qiang Li, Mark A Bayfield, Richard J Maraia, Kunxin Luo, Qiang Zhou
    posted to differentiation drosophila homolog human la pips polymerase premrna protein ptefb rna snrna snrnp transcription tumorigenesis nucleus by jonathanjacobs on 2008-03-31 18:13:35 as ** along with 1 group The La Autoantigen.
  • Novel CNBP- and La-based translation control systems for mammalian cells.
    Biotechnol Bioeng, Vol. 81, No. 1. (5 January 2003), pp. 1-12.
    by S Schlatter, M Fussenegger
    posted to initiation ires la mammalian microscopy mrna plasmid promoter protein ribosome structure top transcription translation utr xenopus genes by jonathanjacobs on 2008-03-31 16:43:51 as ** along with 1 person evaf
  • Was the initiation of translation in early eukaryotes IRES-driven?
    Trends in Biochemical Sciences, Vol. 33, No. 2. (February 2008), pp. 58-64.
    by Greco Hernandez
    posted to eukaryote evolution hypothesis intron ires mrna ribosome structure translation viral by jonathanjacobs on 2008-03-31 16:40:19 as ** along with 1 group Molecular Biology
  • Identification of Lhp1p-associated RNAs by microarray analysis in Saccharomyces cerevisiae reveals association with coding and noncoding RNAs
    Proceedings of the National Academy of Sciences, Vol. 101, No. 2. (13 January 2004), pp. 434-439.
    by Maki Inada, Christine Guthrie
    posted to genomics la mrna polymerase protein ribosome rna saccharomyces transcription translation eukaryote trna lhp1 by jonathanjacobs on 2008-03-31 16:37:55 as ** along with 1 group The La Autoantigen.
  • Proteomic analysis of reaper 5' untranslated region-interacting factors isolated by tobramycin affinity-selection reveals a role for La antigen in reaper mRNA translation
    PROTEOMICS, Vol. 5, No. 6. (2005), pp. 1645-1655.
    by Paula Vazquez-Pianzola, Henning Urlaub, Rolando Rivera-Pomar
    posted to la rna translation utr by jonathanjacobs on 2008-03-31 16:31:55 as read along with 1 group The La Autoantigen.
  • RACK1 mRNA translation is regulated via a rapamycin-sensitive pathway and coordinated with ribosomal protein synthesis
    FEBS Letters, Vol. 579, No. 25. (24 October 2005), pp. 5517-5520.
    by Fabrizio Loreni, Valentina Iadevaia, Elisa Tino, Sara Caldarola, Francesco Amaldi
    posted to mrna ribosome top translation by jonathanjacobs on 2008-03-31 16:28:40 as ***
  • Identification of secondary structure in the 5′-untranslated region of the human adrenomedullin mRNA with implications for the regulation of mRNA translation
    Oncogene, Vol. aop, No. current.
    by F Brenet, N Dussault, C Delfino, F Boudouresque, O Chinot, PM Martin, LH Ouafik
    posted to mrna protein rna structure translation utr by jonathanjacobs on 2008-03-31 16:27:23 as **
  • La protein has a positive effect on the translation of TOP mRNAs in vivo
    Nucl. Acids Res., Vol. 28, No. 15. (1 August 2000), pp. 2927-2934.
    by Claudia Crosio, Pietro P Boyl, Fabrizio Loreni, Paola Pierandrei-Amaldi, Francesco Amaldi
    posted to hypothesis la mrna oligopyrimidine plasmid protein ribosome translation utr vertebrates xenopus genes by jonathanjacobs on 2008-03-31 16:07:08 as **** along with 1 group The La Autoantigen.
  • RNA Interference-Mediated Silencing of the Polo-like Kinase 1 Gene Enhances Chemosensitivity to Gemcitabine in Pancreatic Adenocarcinoma Cells
    Journal of Cellular and Molecular Medicine, Vol. 0, No. ja. (0)
    by YU Chunzhao, Xinhua Zhang, SUN Gu, GUO Xirong, LI Huabing, YOU Yongping, Kevin Gardner, YUAN Depei, XU Zekuan, DU Qing, DAI Cengcai, QIAN Zhuyin, Kuirong Jiang, ZHU Yi, Jonathan L Jacobs, Qingdi Q Li, MIAO Yi
    posted to apoptosis biology cancer genes protein rna rnai by jonathanjacobs on 2008-03-28 05:16:42 as read
  • Transcriptional Networks Inferred from Molecular Signatures of Breast Cancer
    Am J Pathol, Vol. 172, No. 2. (1 February 2008), pp. 495-509.
    by Ron Tongbai, Gila Idelman, Silje H Nordgard, Wenwu Cui, Jonathan L Jacobs, Cynthia M Haggerty, Stephen J Chanock, Anne-Lise Borresen-Dale, Gary Livingston, Patrick Shaunessy, Chih-Hung Chiang, Vessela N Kristensen, Sven Bilke, Kevin Gardner
    posted to genomics genes cancer computational genetics molecular nucleus promoter transcription by jonathanjacobs on 2008-03-28 05:14:48 as read
  • A Three-Stemmed mRNA Pseudoknot in the SARS Coronavirus Frameshift Signal
    PLoS Biology, Vol. 3, No. 6. (1 June 2005), e172.
    by Ewan P Plant, Gabriela C Pérez-Alvarado, Jonathan L Jacobs, Bani Mukhopadhyay, Mirko Hennig, Jonathan D Dinman
    posted to genetics coronavirus genomics computational database frameshift genbank motif mrna mutagenesis mutation phylogenetic predicted protein pseudoknot ribosome rna structure viral by jonathanjacobs on 2008-03-28 04:27:36 as ** along with 1 person physics
  • Identification of functional, endogenous programmed 1 ribosomal frameshift signals in the genome of Saccharomyces cerevisiae
    Nucleic Acids Research, Vol. 35, No. 1. (January 2007), pp. 165-174.
    by Jacobs, L Jonathan, Belew, T Ashton, Rakauskaite, Rasa, Dinman, D Jonathan
    posted to genomics cerevisiae codon computational database frameshift laboratory mrna pathway protein pseudoknot ribosome saccharomyces statistics transcription translation virus yeast by jonathanjacobs on 2008-03-28 04:26:08 as read
  • A programmed -1 ribosomal frameshift signal can function as a cis-acting mRNA destabilizing element
    Nucl. Acids Res., Vol. 32, No. 2. (3 February 2004), pp. 784-790.
    by Ewan P Plant, Pinger Wang, Jonathan L Jacobs, Jonathan D Dinman
    posted to genomics genes frameshift codon consensus hypothesis mrna mutation nmd polycistronic ribosome transcription translation virus yeast by jonathanjacobs on 2008-03-28 04:22:52 as read
  • The 9-A solution: how mRNA pseudoknots promote efficient programmed -1 ribosomal frameshifting.
    RNA, Vol. 9, No. 2. (February 2003), pp. 168-174.
    by EP Plant, KL Jacobs, JW Harger, A Meskauskas, JL Jacobs, JL Baxter, AN Petrov, JD Dinman
    posted to genetics codon frameshift molecular mrna pseudoknot ribosome rna trna by jonathanjacobs on 2008-03-28 04:21:39 as read along with 2 people physics KristiMuldoonJacobs
  • Recognition of Nascent RNA by the Human La Antigen: Conserved and Divergent Features of Structure and Function
    Mol. Cell. Biol., Vol. 21, No. 2. (15 January 2001), pp. 367-379.
    by Richard J Maraia, Robert V Intine
    posted to eukaryote apoptosis chaperone evolution la motif mrna phosphoprotein protein rna snrnas structure transcription translation trna virus yeast by jonathanjacobs on 2008-03-27 18:37:20 as *** along with 1 person JamesIben
  • Ribosomal Protein S25 mRNA Partners with MTF-1 and La to Provide a p53-mediated Mechanism for Survival or Death
    J. Biol. Chem., Vol. 277, No. 6. (1 February 2002), pp. 4147-4151.
    by Tadepalli Adilakshmi, Roney O Laine
    posted to apoptosis cytoplasm genes la mrna nucleus pathway protein regulation ribosome starvation stress by jonathanjacobs on 2008-03-27 18:34:41 as read
  • BCR/ABL activates mdm2 mRNA translation via the La antigen
    Cancer Cell, Vol. 3, No. 2. (February 2003), pp. 145-160.
    by Rossana Trotta, Tatiana Vignudelli, Olivia Candini, Robert V Intine, Luisa Pecorari, Clara Guerzoni, Giorgia Santilli, Mike W Byrom, Silvia