On the specificity of DNA-protein interactions.

by: PH von Hippel, OG Berg

Proc Natl Acad Sci U S A, Vol. 83, No. 6. (March 1986), pp. 1608-1612.

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Abstract

In this paper we summarize the various factors that must be considered in establishing the operational specificity of the binding of a protein regulator of gene expression to a DNA target site. We consider informational (combinatorial) aspects of binding-site specification, actual recognition mechanisms, and the thermodynamics of target-site selection against a background of competing pseudospecific and non-(sequence)-specific DNA binding sites. The results provide insight into the design, specification, and possibly the evolution of regulatory proteins and their chromosomal binding targets, as well as into practical aspects of the design of regulatory-protein isolation schemes and physicochemical regulatory considerations in vivo.

@article{citeulike:1776803, abstract = {In this paper we summarize the various factors that must be considered in establishing the operational specificity of the binding of a protein regulator of gene expression to a DNA target site. We consider informational (combinatorial) aspects of binding-site specification, actual recognition mechanisms, and the thermodynamics of target-site selection against a background of competing pseudospecific and non-(sequence)-specific DNA binding sites. The results provide insight into the design, specification, and possibly the evolution of regulatory proteins and their chromosomal binding targets, as well as into practical aspects of the design of regulatory-protein isolation schemes and physicochemical regulatory considerations in vivo.}, author = {von Hippel, P. H. and Berg, O. G. }, citeulike-article-id = {1776803}, doi = {http://dx.doi.org/10.1073/pnas.83.6.1608}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {dna\_binding, gene\_regulation, thermodynamics}, month = {March}, number = {6}, pages = {1608--1612}, posted-at = {2008-04-01 20:34:15}, priority = {2}, title = {On the specificity of DNA-protein interactions.}, url = {http://dx.doi.org/10.1073/pnas.83.6.1608}, volume = {83}, year = {1986} }

RIS record

TY - JOUR ID - citeulike:1776803 L3 - citeulike-article-id:1776803 TI - On the specificity of DNA-protein interactions. JF - Proc Natl Acad Sci U S A VL - 83 IS - 6 SP - 1608 EP - 1612 SN - 0027-8424 N2 - In this paper we summarize the various factors that must be considered in establishing the operational specificity of the binding of a protein regulator of gene expression to a DNA target site. We consider informational (combinatorial) aspects of binding-site specification, actual recognition mechanisms, and the thermodynamics of target-site selection against a background of competing pseudospecific and non-(sequence)-specific DNA binding sites. The results provide insight into the design, specification, and possibly the evolution of regulatory proteins and their chromosomal binding targets, as well as into practical aspects of the design of regulatory-protein isolation schemes and physicochemical regulatory considerations in vivo. KW - dna_binding KW - gene_regulation KW - thermodynamics AU - von Hippel, PH AU - Berg, OG PY - 1986/03// UR - http://dx.doi.org/10.1073/pnas.83.6.1608 ER -

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