Purification of a monocyte chemotactic factor secreted by nonhuman primate vascular cells in culture.

@article{citeulike:1590155, abstract = {A protein chemotactic for peripheral blood monocytes (SMC-CF) of potential importance in their recruitment to the arterial intima in atherogenesis was purified from serum-free medium conditioned by cultured baboon aortic medial smooth muscle cells. The purification of SMC-CF was monitored by a filter assay using human peripheral blood mononuclear cells and was achieved by batch separation on a cation-exchange gel followed by gel permeation chromatography, ion-exchange high-performance liquid chromatography (HPLC), and reversed-phase HPLC. The overall recovery was approximately 10\% of the initial activity and yielded 0.5-1 microgram of SMC-CF/L of conditioned medium. On analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis, SMC-CF migrated as a monomeric protein with an apparent molecular weight of 14,500. A dose-dependent relationship was observed between SMC-CF concentration and monocyte chemotactic activity, with maximal and half-maximal biologic activity being observed at approximately 5 and 0.1 nM, respectively. Cultured baboon aortic smooth muscle cells also express the genes for both the A and B polypeptide chains of platelet-derived growth factor, which has been reported to be chemotactic for blood monocytes and neutrophils [Deuel, T. F., Senior, R. M., Huang, J. S., \& Griffin, G. L. (1982) J. Clin. Invest. 69, 1046-1049]. Amino acid composition analyses indicate that SMC-CF is not derived either from polypeptide chain of this growth factor or from certain potentially chemotactic connective tissue proteins.}, address = {Department of Pathology, University of Texas Health Science Center, San Antonio 78284.}, author = {Valente, A. J. and Graves, D. T. and Vialle-Valentin, C. E. and Delgado, R. and Schwartz, C. J. }, citeulike-article-id = {1590155}, issn = {0006-2960}, journal = {Biochemistry}, keywords = {ccl2, ccr2, chemokine, macrophage}, month = {May}, number = {11}, pages = {4162--4168}, posted-at = {2007-08-24 16:37:57}, priority = {0}, title = {Purification of a monocyte chemotactic factor secreted by nonhuman primate vascular cells in culture.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3415979}, volume = {27}, year = {1988} }

TY - JOUR ID - citeulike:1590155 L3 - citeulike-article-id:1590155 TI - Purification of a monocyte chemotactic factor secreted by nonhuman primate vascular cells in culture. JF - Biochemistry VL - 27 IS - 11 SP - 4162 EP - 4168 AD - Department of Pathology, University of Texas Health Science Center, San Antonio 78284. SN - 0006-2960 N2 - A protein chemotactic for peripheral blood monocytes (SMC-CF) of potential importance in their recruitment to the arterial intima in atherogenesis was purified from serum-free medium conditioned by cultured baboon aortic medial smooth muscle cells. The purification of SMC-CF was monitored by a filter assay using human peripheral blood mononuclear cells and was achieved by batch separation on a cation-exchange gel followed by gel permeation chromatography, ion-exchange high-performance liquid chromatography (HPLC), and reversed-phase HPLC. The overall recovery was approximately 10% of the initial activity and yielded 0.5-1 microgram of SMC-CF/L of conditioned medium. On analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis, SMC-CF migrated as a monomeric protein with an apparent molecular weight of 14,500. A dose-dependent relationship was observed between SMC-CF concentration and monocyte chemotactic activity, with maximal and half-maximal biologic activity being observed at approximately 5 and 0.1 nM, respectively. Cultured baboon aortic smooth muscle cells also express the genes for both the A and B polypeptide chains of platelet-derived growth factor, which has been reported to be chemotactic for blood monocytes and neutrophils [Deuel, T. F., Senior, R. M., Huang, J. S., & Griffin, G. L. (1982) J. Clin. Invest. 69, 1046-1049]. Amino acid composition analyses indicate that SMC-CF is not derived either from polypeptide chain of this growth factor or from certain potentially chemotactic connective tissue proteins. KW - ccl2 KW - ccr2 KW - chemokine KW - macrophage AU - Valente, AJ AU - Graves, DT AU - Vialle-Valentin, CE AU - Delgado, R AU - Schwartz, CJ PY - 1988/05/31/ UR - http://view.ncbi.nlm.nih.gov/pubmed/3415979 ER -