Domain-wide regulation of gene expression in the human genome

@article{citeulike:1620693, abstract = {Transcription factor complexes bind to regulatory sequences of genes, providing a system of individual expression regulation. Targets of distinct transcription factors usually map throughout the genome, without clustering. Nevertheless, highly and weakly expressed genes do cluster in separate chromosomal domains with an average size of 8090 genes. We therefore asked whether, besides transcription factors, an additional level of gene expression regulation exists that acts on chromosomal domains. Here we show that identical green fluorescent protein (GFP) reporter constructs integrated at 90 different chromosomal positions obtain expression levels that correspond to the activity of the domains of integration. These domains are up to 80 genes long and can exert an eightfold effect on the expression levels of integrated genes. 3D-FISH shows that active domains of integration have a more open chromatin structure than integration domains with weak activity. These results reveal a novel domain-wide regulatory mechanism that, together with transcription factors, exerts a dual control over gene transcription. 10.1101/gr.6276007}, author = {Gierman, Hinco J. and Indemans, Mireille H. and Koster, Jan and Goetze, Sandra and Seppen, Jurgen and Geerts, Dirk and van Driel, Roel and Versteeg, Rogier }, citeulike-article-id = {1620693}, doi = {http://dx.doi.org/10.1101/gr.6276007}, journal = {Genome Res.}, keywords = {genome-organization, paper08}, month = {September}, number = {9}, pages = {1286--1295}, posted-at = {2008-07-24 14:05:06}, priority = {5}, title = {Domain-wide regulation of gene expression in the human genome}, url = {http://dx.doi.org/10.1101/gr.6276007}, volume = {17}, year = {2007} }

TY - JOUR ID - citeulike:1620693 L3 - citeulike-article-id:1620693 TI - Domain-wide regulation of gene expression in the human genome JF - Genome Res. VL - 17 IS - 9 SP - 1286 EP - 1295 N2 - Transcription factor complexes bind to regulatory sequences of genes, providing a system of individual expression regulation. Targets of distinct transcription factors usually map throughout the genome, without clustering. Nevertheless, highly and weakly expressed genes do cluster in separate chromosomal domains with an average size of 8090 genes. We therefore asked whether, besides transcription factors, an additional level of gene expression regulation exists that acts on chromosomal domains. Here we show that identical green fluorescent protein (GFP) reporter constructs integrated at 90 different chromosomal positions obtain expression levels that correspond to the activity of the domains of integration. These domains are up to 80 genes long and can exert an eightfold effect on the expression levels of integrated genes. 3D-FISH shows that active domains of integration have a more open chromatin structure than integration domains with weak activity. These results reveal a novel domain-wide regulatory mechanism that, together with transcription factors, exerts a dual control over gene transcription. 10.1101/gr.6276007 KW - genome-organization KW - paper08 AU - Gierman, Hinco AU - Indemans, Mireille AU - Koster, Jan AU - Goetze, Sandra AU - Seppen, Jurgen AU - Geerts, Dirk AU - van Driel, Roel AU - Versteeg, Rogier PY - 2007/09/01/ UR - http://dx.doi.org/10.1101/gr.6276007 ER -