Effect of melatonin treatment on oxygen consumption by rat liver mitochondria.

@article{citeulike:666947, abstract = {The objective of this study was to examine the in vivo effect of melatonin on rat mitochondrial liver respiration. Two experiments were performed: For experiment 1, adult male rats received melatonin in the drinking water (16 or 50 microg/ml) or vehicle during 45 days. For experiment 2, rats received melatonin in the drinking water (50 microg/ml) for 45 days, or the same amount for 30 days followed by a 15 day-withdrawal period. At sacrifice, a liver mitochondrial fraction was prepared and oxygen consumption was measured polarographically in the presence of excess concentration of DL-3-beta-hydroxybutyrate or L-succinate. Melatonin treatment decreased Krebs' cycle substrate-induced respiration significantly at both examined doses. The stimulation of mitochondrial respiration caused by excess concentration of substrate recovered after melatonin withdrawal. Basal state 4 respiration was not modified by melatonin. Melatonin, by curtailing overstimulation of cellular respiration caused by excess Krebs' cycle substrates, can protect the mitochondria from oxidative damage.}, address = {Departamento de Fisiolog\'{i}a and Hospital de Cl\'{i}nicas, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.}, author = {Reyes-Toso, C F F. and Rebagliati, I R R. and Ricci, C R R. and Linares, L M M. and Albornoz, L E E. and Cardinali, D P P. and Zaninovich, A }, citeulike-article-id = {666947}, issn = {0939-4451}, journal = {Amino Acids}, keywords = {aging, antioxidants, glutathione, melatonin, mitochondria, ros}, month = {March}, posted-at = {2006-05-24 00:59:04}, priority = {2}, title = {Effect of melatonin treatment on oxygen consumption by rat liver mitochondria.}, url = {http://dx.doi.org/10.1007/s00726-005-0280-z}, year = {2006} }

TY - JOUR ID - citeulike:666947 L3 - citeulike-article-id:666947 TI - Effect of melatonin treatment on oxygen consumption by rat liver mitochondria. JF - Amino Acids AD - Departamento de Fisiología and Hospital de Clínicas, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina. SN - 0939-4451 N2 - The objective of this study was to examine the in vivo effect of melatonin on rat mitochondrial liver respiration. Two experiments were performed: For experiment 1, adult male rats received melatonin in the drinking water (16 or 50 microg/ml) or vehicle during 45 days. For experiment 2, rats received melatonin in the drinking water (50 microg/ml) for 45 days, or the same amount for 30 days followed by a 15 day-withdrawal period. At sacrifice, a liver mitochondrial fraction was prepared and oxygen consumption was measured polarographically in the presence of excess concentration of DL-3-beta-hydroxybutyrate or L-succinate. Melatonin treatment decreased Krebs' cycle substrate-induced respiration significantly at both examined doses. The stimulation of mitochondrial respiration caused by excess concentration of substrate recovered after melatonin withdrawal. Basal state 4 respiration was not modified by melatonin. Melatonin, by curtailing overstimulation of cellular respiration caused by excess Krebs' cycle substrates, can protect the mitochondria from oxidative damage. KW - aging KW - antioxidants KW - glutathione KW - melatonin KW - mitochondria KW - ros AU - Reyes-Toso, C F AU - Rebagliati, I R AU - Ricci, C R AU - Linares, L M AU - Albornoz, L E AU - Cardinali, D P AU - Zaninovich, A PY - 2006/03/24/ UR - http://dx.doi.org/10.1007/s00726-005-0280-z ER -