'The 39 steps' in gene expression profiling: critical issues and proposed best practices for microarray experiments.

@article{citeulike:934250, abstract = {Gene expression microarrays have been used widely to address increasingly complex biological questions and to produce an unprecedented amount of data, but have yet to realize their full potential. The interpretation of microarray data remains a major challenge because of the complexity of the underlying biological networks. To gather meaningful expression data, it is crucial to develop standardized approaches for vigilant study design, controlled annotation of resources, careful quality control of experiments, robust statistics, and data registration and storage. This article reviews the steps needed in the design and execution of valid microarray experiments so that global gene expression data can play a major role in the pursuit of future biological discoveries that will impact drug development.}, address = {Array s/IMAGE, Genexpress, Functional Genomics and Systems Biology for Health, LGN UMR 7091, CNRS and Pierre and Marie Curie University, Paris VI, Villejuif, France. imbeaud@vjf.cnrs.fr}, author = {Imbeaud, S. and Auffray, C. }, citeulike-article-id = {934250}, doi = {http://dx.doi.org/10.1016/S1359-6446(05)03565-8}, issn = {1359-6446}, journal = {Drug Discov Today}, keywords = {microarray, microarray\_functional\_analysis}, month = {September}, number = {17}, pages = {1175--1182}, posted-at = {2008-01-11 14:31:30}, priority = {0}, title = {'The 39 steps' in gene expression profiling: critical issues and proposed best practices for microarray experiments.}, url = {http://dx.doi.org/10.1016/S1359-6446(05)03565-8}, volume = {10}, year = {2005} }

TY - JOUR ID - citeulike:934250 L3 - citeulike-article-id:934250 TI - 'The 39 steps' in gene expression profiling: critical issues and proposed best practices for microarray experiments. JF - Drug Discov Today VL - 10 IS - 17 SP - 1175 EP - 1182 AD - Array s/IMAGE, Genexpress, Functional Genomics and Systems Biology for Health, LGN UMR 7091, CNRS and Pierre and Marie Curie University, Paris VI, Villejuif, France. imbeaud@vjf.cnrs.fr SN - 1359-6446 N2 - Gene expression microarrays have been used widely to address increasingly complex biological questions and to produce an unprecedented amount of data, but have yet to realize their full potential. The interpretation of microarray data remains a major challenge because of the complexity of the underlying biological networks. To gather meaningful expression data, it is crucial to develop standardized approaches for vigilant study design, controlled annotation of resources, careful quality control of experiments, robust statistics, and data registration and storage. This article reviews the steps needed in the design and execution of valid microarray experiments so that global gene expression data can play a major role in the pursuit of future biological discoveries that will impact drug development. KW - microarray KW - microarray_functional_analysis AU - Imbeaud, S AU - Auffray, C PY - 2005/09/01/ UR - http://dx.doi.org/10.1016/S1359-6446(05)03565-8 ER -