MicroRNA1 influences cardiac differentiation in Drosophila and regulates Notch signaling.

@article{citeulike:781401, abstract = {MicroRNAs (miRNAs) are genomically encoded small RNAs that hybridize with messenger RNAs, resulting in degradation or translational inhibition of targeted transcripts. The potential for miRNAs to regulate cell-lineage determination or differentiation from pluripotent progenitor or stem cells is unknown. Here, we show that microRNA1 (miR-1) is an ancient muscle-specific gene conserved in sequence and expression in Drosophila. Drosophila miR-1 (dmiR-1) is regulated through a serum response factor-like binding site in cardiac progenitor cells. Loss- and gain-of-function studies demonstrated a role for dmiR-1 in modulating cardiogenesis and in maintenance of muscle-gene expression. We provide in vivo evidence that dmiR-1 targets transcripts encoding the Notch ligand Delta, providing a potential mechanism for the expansion of cardiac and muscle progenitor cells and failure of progenitor cell differentiation in some dmiR-1 mutants. These findings demonstrate that dmiR-1 may "fine-tune" critical steps involved in differentiation of cardiac and somatic muscle progenitors and targets a pathway required for progenitor cell specification and asymmetric cell division.}, address = {Gladstone Institute of Cardiovascular Disease and Department of Pediatrics, University of California, 1650 Owens Street, San Francisco, CA 94158.}, author = {Kwon, C. and Han, Z. and Olson, E. N. and Srivastava, D. }, citeulike-article-id = {781401}, doi = {http://dx.doi.org/10.1073/pnas.0509535102}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {microrna}, month = {December}, number = {52}, pages = {18986--18991}, posted-at = {2007-10-25 12:20:47}, priority = {2}, title = {MicroRNA1 influences cardiac differentiation in Drosophila and regulates Notch signaling.}, url = {http://dx.doi.org/10.1073/pnas.0509535102}, volume = {102}, year = {2005} }

TY - JOUR ID - citeulike:781401 L3 - citeulike-article-id:781401 TI - MicroRNA1 influences cardiac differentiation in Drosophila and regulates Notch signaling. JF - Proc Natl Acad Sci U S A VL - 102 IS - 52 SP - 18986 EP - 18991 AD - Gladstone Institute of Cardiovascular Disease and Department of Pediatrics, University of California, 1650 Owens Street, San Francisco, CA 94158. SN - 0027-8424 N2 - MicroRNAs (miRNAs) are genomically encoded small RNAs that hybridize with messenger RNAs, resulting in degradation or translational inhibition of targeted transcripts. The potential for miRNAs to regulate cell-lineage determination or differentiation from pluripotent progenitor or stem cells is unknown. Here, we show that microRNA1 (miR-1) is an ancient muscle-specific gene conserved in sequence and expression in Drosophila. Drosophila miR-1 (dmiR-1) is regulated through a serum response factor-like binding site in cardiac progenitor cells. Loss- and gain-of-function studies demonstrated a role for dmiR-1 in modulating cardiogenesis and in maintenance of muscle-gene expression. We provide in vivo evidence that dmiR-1 targets transcripts encoding the Notch ligand Delta, providing a potential mechanism for the expansion of cardiac and muscle progenitor cells and failure of progenitor cell differentiation in some dmiR-1 mutants. These findings demonstrate that dmiR-1 may "fine-tune" critical steps involved in differentiation of cardiac and somatic muscle progenitors and targets a pathway required for progenitor cell specification and asymmetric cell division. KW - microrna AU - Kwon, C AU - Han, Z AU - Olson, EN AU - Srivastava, D PY - 2005/12/27/ UR - http://dx.doi.org/10.1073/pnas.0509535102 ER -