Microarray analysis on Runx2-deficient mouse embryos reveals novel Runx2 functions and target genes during intramembranous and endochondral bone formation.

@article{citeulike:700996, abstract = {A major challenge in developmental biology is to correlate genome-wide gene expression modulations with developmental processes in vivo. In this study, we analyzed the role of Runx2 during intramembranous and endochondral bone development, by comparing gene expression profiles in 14.5 dpc wild-type and Runx2 (-/-) mice. A total of 1277, 606 and 492 transcripts were found to be significantly modulated by Runx2 in calvaria, forelimbs and hindlimbs, respectively. Bioinformatics analysis indicated that Runx2 not only controls the processes of osteoblast differentiation and chondrocyte maturation, but may also play a role in axon formation and hematopoietic cell commitment during bone development. A total of 41 genes are affected by the Runx2 deletion in both intramembranous and endochondral bone, indicating common pathways between these two developmental modes of bone formation. In addition, we identified genes that are specifically involved in endochondral ossification. In conclusion, our data show that a comparative genome-wide expression analysis of wild-type and mutant mouse models allows the examination of mutant phenotypes in complex tissues.}, address = {Department of Applied Biology FNWI, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.}, author = {Vaes, Bart L T L. and Ducy, Patricia and Sijbers, Anneke M M. and Hendriks, Jos\'{e} M A M. and van Someren, Eugene P P. and de Jong, Nanning G G. and van den Heuvel, Edwin R R. and Olijve, Wiebe and van Zoelen, Everardus J J J. and Dechering, Koen J J. }, citeulike-article-id = {700996}, doi = {10.1016/j.bone.2006.04.024}, issn = {8756-3282}, journal = {Bone}, keywords = {chondrocyte, microarray}, month = {June}, posted-at = {2008-02-25 15:53:40}, priority = {5}, title = {Microarray analysis on Runx2-deficient mouse embryos reveals novel Runx2 functions and target genes during intramembranous and endochondral bone formation.}, url = {http://dx.doi.org/10.1016/j.bone.2006.04.024}, year = {2006} }

TY - JOUR ID - citeulike:700996 L3 - citeulike-article-id:700996 TI - Microarray analysis on Runx2-deficient mouse embryos reveals novel Runx2 functions and target genes during intramembranous and endochondral bone formation. JF - Bone AD - Department of Applied Biology FNWI, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands. SN - 8756-3282 N2 - A major challenge in developmental biology is to correlate genome-wide gene expression modulations with developmental processes in vivo. In this study, we analyzed the role of Runx2 during intramembranous and endochondral bone development, by comparing gene expression profiles in 14.5 dpc wild-type and Runx2 (-/-) mice. A total of 1277, 606 and 492 transcripts were found to be significantly modulated by Runx2 in calvaria, forelimbs and hindlimbs, respectively. Bioinformatics analysis indicated that Runx2 not only controls the processes of osteoblast differentiation and chondrocyte maturation, but may also play a role in axon formation and hematopoietic cell commitment during bone development. A total of 41 genes are affected by the Runx2 deletion in both intramembranous and endochondral bone, indicating common pathways between these two developmental modes of bone formation. In addition, we identified genes that are specifically involved in endochondral ossification. In conclusion, our data show that a comparative genome-wide expression analysis of wild-type and mutant mouse models allows the examination of mutant phenotypes in complex tissues. KW - chondrocyte KW - microarray AU - Vaes, Bart L T AU - Ducy, Patricia AU - Sijbers, Anneke M AU - Hendriks, José M A AU - van Someren, Eugene P AU - de Jong, Nanning G AU - van den Heuvel, Edwin R AU - Olijve, Wiebe AU - van Zoelen, Everardus J J AU - Dechering, Koen J PY - 2006/06/13/ UR - http://dx.doi.org/10.1016/j.bone.2006.04.024 ER -