MicroRNAs direct rapid deadenylation of mRNA.

by: Ligang Wu, Jihua Fan, Joel G G Belasco

Proc Natl Acad Sci U S A (22 February 2006)

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Abstract

MicroRNAs (miRNAs) are ubiquitous regulators of eukaryotic gene expression. In addition to repressing translation, miRNAs can down-regulate the concentration of mRNAs that contain elements to which they are imperfectly complementary. Using miR-125b and let-7 as representative miRNAs, we show that in mammalian cells this reduction in message abundance is a consequence of accelerated deadenylation, which leads to rapid mRNA decay. The ability of miRNAs to expedite poly(A) removal does not result from decreased translation; nor does translational repression by miRNAs require a poly(A) tail, a 3' histone stem-loop being an effective substitute. These findings suggest that miRNAs use two distinct posttranscriptional mechanisms to down-regulate gene expression.

@article{citeulike:520112, abstract = {MicroRNAs (miRNAs) are ubiquitous regulators of eukaryotic gene expression. In addition to repressing translation, miRNAs can down-regulate the concentration of mRNAs that contain elements to which they are imperfectly complementary. Using miR-125b and let-7 as representative miRNAs, we show that in mammalian cells this reduction in message abundance is a consequence of accelerated deadenylation, which leads to rapid mRNA decay. The ability of miRNAs to expedite poly(A) removal does not result from decreased translation; nor does translational repression by miRNAs require a poly(A) tail, a 3' histone stem-loop being an effective substitute. These findings suggest that miRNAs use two distinct posttranscriptional mechanisms to down-regulate gene expression.}, address = {Skirball Institute of Biomolecular Medicine and Department of Microbiology, New York University School of Medicine, New York, NY 10016.}, author = {Wu, Ligang and Fan, Jihua and Belasco, Joel G G. }, citeulike-article-id = {520112}, doi = {10.1073/pnas.0510928103}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {deadenylation, microrna}, month = {February}, posted-at = {2007-12-10 18:04:10}, priority = {0}, title = {MicroRNAs direct rapid deadenylation of mRNA.}, url = {http://dx.doi.org/10.1073/pnas.0510928103}, year = {2006} }

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TY - JOUR ID - citeulike:520112 L3 - citeulike-article-id:520112 TI - MicroRNAs direct rapid deadenylation of mRNA. JF - Proc Natl Acad Sci U S A AD - Skirball Institute of Biomolecular Medicine and Department of Microbiology, New York University School of Medicine, New York, NY 10016. SN - 0027-8424 N2 - MicroRNAs (miRNAs) are ubiquitous regulators of eukaryotic gene expression. In addition to repressing translation, miRNAs can down-regulate the concentration of mRNAs that contain elements to which they are imperfectly complementary. Using miR-125b and let-7 as representative miRNAs, we show that in mammalian cells this reduction in message abundance is a consequence of accelerated deadenylation, which leads to rapid mRNA decay. The ability of miRNAs to expedite poly(A) removal does not result from decreased translation; nor does translational repression by miRNAs require a poly(A) tail, a 3' histone stem-loop being an effective substitute. These findings suggest that miRNAs use two distinct posttranscriptional mechanisms to down-regulate gene expression. KW - deadenylation KW - microrna AU - Wu, Ligang AU - Fan, Jihua AU - Belasco, Joel G PY - 2006/02/22/ UR - http://dx.doi.org/10.1073/pnas.0510928103 ER -

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