Identification of genes responsible for osteoblast differentiation from human mesodermal progenitor cells

@article{citeulike:2429891, abstract = {Single human bone marrow-derived mesodermal progenitor cells (MPCs) differentiate into osteoblasts, chondrocytes, adipocytes, myocytes, and endothelial cells. To identify genes involved in the commitment of MPCs to osteoblasts we examined the expressed gene profile of undifferentiated MPCs and MPCs induced to the osteoblast lineage for 1-7 days by cDNA microarray analysis. As expected, growth factor, hormone, and signaling pathway genes known to be involved in osteogenesis were activated during differentiation. In addition, 41 transcription factors (TFs) were differentially expressed over time, including TFs with known roles in osteoblast differentiation and TFs not known to be involved in osteoblast differentiation. As the latter group of TFs coclustered with osteogenesis-specific TFs, they may play a role in osteoblast differentiation. When we compared the gene expression profile of MPCs induced to differentiate to chondroblasts and osteoblasts, significant differences in the nature and/or timing of gene activation were seen. These studies indicate that in vitro differentiation cultures in which MPCs are induced to one of multiple cell fates should be very useful for defining signals important for lineage-specific differentiation. 10.1073/pnas.0532693100}, author = {Qi, Huilin and Aguiar, Dean J. and Williams, Shelly M. and La Pean, Alison and Pan, Wei and Verfaillie, Catherine M. }, citeulike-article-id = {2429891}, doi = {http://dx.doi.org/10.1073/pnas.0532693100}, journal = {Proceedings of the National Academy of Sciences}, keywords = {bone\_formation, microarray}, month = {March}, number = {6}, pages = {3305--3310}, posted-at = {2008-02-26 14:51:47}, priority = {2}, title = {Identification of genes responsible for osteoblast differentiation from human mesodermal progenitor cells}, url = {http://dx.doi.org/10.1073/pnas.0532693100}, volume = {100}, year = {2003} }

TY - JOUR ID - citeulike:2429891 L3 - citeulike-article-id:2429891 TI - Identification of genes responsible for osteoblast differentiation from human mesodermal progenitor cells JF - Proceedings of the National Academy of Sciences VL - 100 IS - 6 SP - 3305 EP - 3310 N2 - Single human bone marrow-derived mesodermal progenitor cells (MPCs) differentiate into osteoblasts, chondrocytes, adipocytes, myocytes, and endothelial cells. To identify genes involved in the commitment of MPCs to osteoblasts we examined the expressed gene profile of undifferentiated MPCs and MPCs induced to the osteoblast lineage for 1-7 days by cDNA microarray analysis. As expected, growth factor, hormone, and signaling pathway genes known to be involved in osteogenesis were activated during differentiation. In addition, 41 transcription factors (TFs) were differentially expressed over time, including TFs with known roles in osteoblast differentiation and TFs not known to be involved in osteoblast differentiation. As the latter group of TFs coclustered with osteogenesis-specific TFs, they may play a role in osteoblast differentiation. When we compared the gene expression profile of MPCs induced to differentiate to chondroblasts and osteoblasts, significant differences in the nature and/or timing of gene activation were seen. These studies indicate that in vitro differentiation cultures in which MPCs are induced to one of multiple cell fates should be very useful for defining signals important for lineage-specific differentiation. 10.1073/pnas.0532693100 KW - bone_formation KW - microarray AU - Qi, Huilin AU - Aguiar, Dean AU - Williams, Shelly AU - La Pean, Alison AU - Pan, Wei AU - Verfaillie, Catherine PY - 2003/03/18/ UR - http://dx.doi.org/10.1073/pnas.0532693100 ER -