Design, construction and performance of the most efficient biomass producing E. coli bacterium

by: Cong T Trinh, Ross Carlson, Aaron Wlaschin, Friedrich Srienc

Metabolic Engineering, Vol. 8, No. 6. (November 2006), pp. 628-638.

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Abstract

Inverse metabolic engineering based on elementary mode analysis was applied to maximize the biomass yield of Escherchia coli MG1655. Elementary mode analysis was previously employed to identify among 1691 possible pathways for cell growth the most efficient pathway with maximum biomass yield. The metabolic network analysis predicted that deletion of only 6 genes reduces the number of possible elementary modes to the most efficient pathway. We have constructed a strain containing these gene deletions and we evaluated its properties in batch and in chemostat growth experiments. The results show that the theoretical predictions are closely matched by the properties of the designed strain.

@article{citeulike:2683782, abstract = {Inverse metabolic engineering based on elementary mode analysis was applied to maximize the biomass yield of Escherchia coli MG1655. Elementary mode analysis was previously employed to identify among 1691 possible pathways for cell growth the most efficient pathway with maximum biomass yield. The metabolic network analysis predicted that deletion of only 6 genes reduces the number of possible elementary modes to the most efficient pathway. We have constructed a strain containing these gene deletions and we evaluated its properties in batch and in chemostat growth experiments. The results show that the theoretical predictions are closely matched by the properties of the designed strain.}, author = {Trinh, Cong T. and Carlson, Ross and Wlaschin, Aaron and Srienc, Friedrich }, citeulike-article-id = {2683782}, doi = {http://dx.doi.org/10.1016/j.ymben.2006.07.006}, journal = {Metabolic Engineering}, keywords = {ecoli\_ppp}, month = {November}, number = {6}, pages = {628--638}, posted-at = {2008-04-18 00:27:51}, priority = {0}, title = {Design, construction and performance of the most efficient biomass producing E. coli bacterium}, url = {http://dx.doi.org/10.1016/j.ymben.2006.07.006}, volume = {8}, year = {2006} }

RIS record

TY - JOUR ID - citeulike:2683782 L3 - citeulike-article-id:2683782 TI - Design, construction and performance of the most efficient biomass producing E. coli bacterium JF - Metabolic Engineering VL - 8 IS - 6 SP - 628 EP - 638 N2 - Inverse metabolic engineering based on elementary mode analysis was applied to maximize the biomass yield of Escherchia coli MG1655. Elementary mode analysis was previously employed to identify among 1691 possible pathways for cell growth the most efficient pathway with maximum biomass yield. The metabolic network analysis predicted that deletion of only 6 genes reduces the number of possible elementary modes to the most efficient pathway. We have constructed a strain containing these gene deletions and we evaluated its properties in batch and in chemostat growth experiments. The results show that the theoretical predictions are closely matched by the properties of the designed strain. KW - ecoli_ppp AU - Trinh, Cong AU - Carlson, Ross AU - Wlaschin, Aaron AU - Srienc, Friedrich PY - 2006/11// UR - http://dx.doi.org/10.1016/j.ymben.2006.07.006 ER -

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