Human Alu RNA Is a Modular Transacting Repressor of mRNA Transcription during Heat Shock.

@article{MARINER2008, abstract = {Noncoding RNAs (ncRNAs) have recently been discovered to regulate mRNA transcription in trans, a role traditionally reserved for proteins. The breadth of ncRNAs as transacting transcriptional regulators and the diversity of signals to which they respond are only now becoming recognized. Here we show that human Alu RNA, transcribed from short interspersed elements (SINEs), is a transacting transcriptional repressor during the cellular heat shock response. Alu RNA blocks transcription by binding RNA polymerase II (Pol II) and entering complexes at promoters in vitro and in human cells. Transcriptional repression by Alu RNA involves two loosely structured domains that are modular, a property reminiscent of classical protein transcriptional regulators. Two other SINE RNAs, human scAlu RNA and mouse B1 RNA, also bind Pol II but do not repress transcription in vitro. These studies provide an explanation for why mouse cells harbor two major classes of SINEs, whereas human cells contain only one.}, address = {Department of Chemistry and Biochemistry, University of Colorado at Boulder, 215 UCB, Boulder, CO 80309-0215, USA.}, author = {Mariner, P. D. and Walters, R. D. and Espinoza, C. A. and Drullinger, L. F. and Wagner, S. D. and Kugel, J. F. and Goodrich, J. A. }, citeulike-article-id = {2570345}, doi = {10.1016/j.molcel.2007.12.013}, issn = {1097-2765}, journal = {Mol Cell}, keywords = {alu, rna}, month = {February}, number = {4}, pages = {499--509}, posted-at = {2008-05-08 15:07:22}, priority = {2}, title = {Human Alu RNA Is a Modular Transacting Repressor of mRNA Transcription during Heat Shock.}, url = {http://dx.doi.org/10.1016/j.molcel.2007.12.013}, volume = {29}, year = {2008} }

TY - JOUR ID - MARINER2008 L3 - citeulike-article-id:2570345 TI - Human Alu RNA Is a Modular Transacting Repressor of mRNA Transcription during Heat Shock. JF - Mol Cell VL - 29 IS - 4 SP - 499 EP - 509 AD - Department of Chemistry and Biochemistry, University of Colorado at Boulder, 215 UCB, Boulder, CO 80309-0215, USA. SN - 1097-2765 N2 - Noncoding RNAs (ncRNAs) have recently been discovered to regulate mRNA transcription in trans, a role traditionally reserved for proteins. The breadth of ncRNAs as transacting transcriptional regulators and the diversity of signals to which they respond are only now becoming recognized. Here we show that human Alu RNA, transcribed from short interspersed elements (SINEs), is a transacting transcriptional repressor during the cellular heat shock response. Alu RNA blocks transcription by binding RNA polymerase II (Pol II) and entering complexes at promoters in vitro and in human cells. Transcriptional repression by Alu RNA involves two loosely structured domains that are modular, a property reminiscent of classical protein transcriptional regulators. Two other SINE RNAs, human scAlu RNA and mouse B1 RNA, also bind Pol II but do not repress transcription in vitro. These studies provide an explanation for why mouse cells harbor two major classes of SINEs, whereas human cells contain only one. KW - alu KW - rna AU - Mariner, PD AU - Walters, RD AU - Espinoza, CA AU - Drullinger, LF AU - Wagner, SD AU - Kugel, JF AU - Goodrich, JA PY - 2008/02/29/ UR - http://dx.doi.org/10.1016/j.molcel.2007.12.013 ER -