Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection.

@article{citeulike:2965792, abstract = {Viral infections induce extensive T cell proliferation in vivo, but the specificity of the majority of the responding T cells has not been defined. To address this issue we used tetramers of MHC class I molecules containing viral peptides to directly visualize antigen-specific CD8 T cells during acute LCMV infection of mice. Based on tetramer binding and two sensitive assays measuring interferon-gamma production at the single-cell level, we found that 50\%-70\% of the activated CD8 T cells were LCMV specific [2 x 10(7) virus-specific cells/spleen]. Following viral clearance, antigen-specific CD8 T cell numbers dropped to 10(6) per spleen and were maintained at this level for the life of the mouse. Upon rechallenge with LCMV, there was rapid expansion of memory T cells, but after infection with the heterologous vaccinia virus there was no detectable change in the numbers of LCMV-specific memory CTL. Therefore, much of the CD8 T cell expansion seen during viral infection represents antigen-specific cells and warrants a revision of our current thinking on the size of the antiviral response.}, address = {Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.}, author = {Murali-Krishna, K. and Altman, J. D. and Suresh, M. and Sourdive, D. J. and Zajac, A. J. and Miller, J. D. and Slansky, J. and Ahmed, R. }, citeulike-article-id = {2965792}, issn = {1074-7613}, journal = {Immunity}, month = {February}, number = {2}, pages = {177--187}, posted-at = {2008-07-05 11:06:42}, priority = {2}, title = {Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9491999}, volume = {8}, year = {1998} }

TY - JOUR ID - citeulike:2965792 L3 - citeulike-article-id:2965792 TI - Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection. JF - Immunity VL - 8 IS - 2 SP - 177 EP - 187 AD - Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA. SN - 1074-7613 N2 - Viral infections induce extensive T cell proliferation in vivo, but the specificity of the majority of the responding T cells has not been defined. To address this issue we used tetramers of MHC class I molecules containing viral peptides to directly visualize antigen-specific CD8 T cells during acute LCMV infection of mice. Based on tetramer binding and two sensitive assays measuring interferon-gamma production at the single-cell level, we found that 50%-70% of the activated CD8 T cells were LCMV specific [2 x 10(7) virus-specific cells/spleen]. Following viral clearance, antigen-specific CD8 T cell numbers dropped to 10(6) per spleen and were maintained at this level for the life of the mouse. Upon rechallenge with LCMV, there was rapid expansion of memory T cells, but after infection with the heterologous vaccinia virus there was no detectable change in the numbers of LCMV-specific memory CTL. Therefore, much of the CD8 T cell expansion seen during viral infection represents antigen-specific cells and warrants a revision of our current thinking on the size of the antiviral response. AU - Murali-Krishna, K AU - Altman, JD AU - Suresh, M AU - Sourdive, DJ AU - Zajac, AJ AU - Miller, JD AU - Slansky, J AU - Ahmed, R PY - 1998/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/9491999 ER -