A new mechanism for coactivation of transcription initiation: repositioning of an activator triggered by the binding of a second activator.

@article{citeulike:2948954, abstract = {The cAMP receptor protein (CRP) and MaIT, the maltose regulon activator, synergistically activate transcription from the E. coli maIKp promoter. The maIKp regulatory region comprises two series of MaIT-binding sites separated by three CRP-binding sites. By combining genetic and biochemical studies, we demonstrate that the promoter-proximal region contains two overlapping sets of three MaIT-binding sites. Occupation of the higher affinity set of sites, which occurs in the absence of CRP, does not lead to malKp activation. In contrast, in the presence of CRP, MalT binds to the lower affinity set of sites and triggers transcription initiation because, unlike the high affinity set, the low affinity set of sites is properly positioned with respect to the Pribnow box. The CRP effect requires the malKp-distal MalT-binding sites. The synergistic action of MalT and CRP therefore relies on MalT repositioning via the formation of a nucleoprotein structure involving the entire regulatory region.}, address = {Unit\'{e} de G\'{e}n\'{e}tique Mol\'{e}culaire URA 1149 du CNRS, Institut Pasteur, Paris, France.}, author = {Richet, E. and Vidal-Ingigliardi, D. and Raibaud, O. }, citeulike-article-id = {2948954}, issn = {0092-8674}, journal = {Cell}, keywords = {bioregulationoverview}, month = {September}, number = {6}, pages = {1185--1195}, posted-at = {2008-07-01 19:02:48}, priority = {2}, title = {A new mechanism for coactivation of transcription initiation: repositioning of an activator triggered by the binding of a second activator.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1913806}, volume = {66}, year = {1991} }

TY - JOUR ID - citeulike:2948954 L3 - citeulike-article-id:2948954 TI - A new mechanism for coactivation of transcription initiation: repositioning of an activator triggered by the binding of a second activator. JF - Cell VL - 66 IS - 6 SP - 1185 EP - 1195 AD - Unité de Génétique Moléculaire URA 1149 du CNRS, Institut Pasteur, Paris, France. SN - 0092-8674 N2 - The cAMP receptor protein (CRP) and MaIT, the maltose regulon activator, synergistically activate transcription from the E. coli maIKp promoter. The maIKp regulatory region comprises two series of MaIT-binding sites separated by three CRP-binding sites. By combining genetic and biochemical studies, we demonstrate that the promoter-proximal region contains two overlapping sets of three MaIT-binding sites. Occupation of the higher affinity set of sites, which occurs in the absence of CRP, does not lead to malKp activation. In contrast, in the presence of CRP, MalT binds to the lower affinity set of sites and triggers transcription initiation because, unlike the high affinity set, the low affinity set of sites is properly positioned with respect to the Pribnow box. The CRP effect requires the malKp-distal MalT-binding sites. The synergistic action of MalT and CRP therefore relies on MalT repositioning via the formation of a nucleoprotein structure involving the entire regulatory region. KW - bioregulationoverview AU - Richet, E AU - Vidal-Ingigliardi, D AU - Raibaud, O PY - 1991/09/20/ UR - http://view.ncbi.nlm.nih.gov/pubmed/1913806 ER -