Antiparallel DNA Double Crossover Molecules As Components for Nanoconstruction

@article{citeulike:2791094, abstract = {Abstract: Double crossover molecules are DNA structures containing two Holliday junctions connected by two double helical arms. There are several types of double crossover molecules, differentiated by the relative orientations of their helix axes, parallel or antiparallel, and by the number of double helical half-turns (even or odd) between the two crossovers. We have examined these molecules from the viewpoint of their potential utility in nanoconstruction. Whereas the parallel double helical molecules are usually not well behaved, we have focused on the antiparallel molecules; antiparallel molecules with an even number of half turns between crossovers (termed DAE molecules) produce a reporter strand when ligated, so these have been characterized in a ligation cyclization assay. In contrast to other molecules that contain branched junctions, we find that these molecules cyclize rarely or not at all. The double crossover molecules cyclize no more readily than the linear molecule containing the same sequence as the ligation domain. We have tested both a conventional DAE molecule and one containing a bulged three-arm branched junction between the crossovers. The conventional DAE molecule appears to be slightly stiffer, but so few cyclic products are obtained in either case that quantitative comparisons are not possible. Thus, it appears that these molecules may be able to serve as the rigid components that are needed to assemble symmetric molecular structures, such as periodic lattices. We suggest that they be combined with DNA triangles and deltahedra in order to accomplish this goal.}, address = {Contribution from the Department of Chemistry, New York University, New York, New York 10003}, author = {Li, X. and Yang, X. and Qi, J. and Seeman, N. C. }, citeulike-article-id = {2791094}, doi = {10.1021/ja960162o}, journal = {J. Am. Chem. Soc.}, keywords = {origami, origami-design}, month = {July}, number = {26}, pages = {6131--6140}, posted-at = {2008-05-12 23:24:54}, priority = {2}, title = {Antiparallel DNA Double Crossover Molecules As Components for Nanoconstruction}, url = {http://dx.doi.org/10.1021/ja960162o}, volume = {118}, year = {1996} }

TY - JOUR ID - citeulike:2791094 L3 - citeulike-article-id:2791094 TI - Antiparallel DNA Double Crossover Molecules As Components for Nanoconstruction JF - J. Am. Chem. Soc. VL - 118 IS - 26 SP - 6131 EP - 6140 AD - Contribution from the Department of Chemistry, New York University, New York, New York 10003 N2 - Abstract: Double crossover molecules are DNA structures containing two Holliday junctions connected by two double helical arms. There are several types of double crossover molecules, differentiated by the relative orientations of their helix axes, parallel or antiparallel, and by the number of double helical half-turns (even or odd) between the two crossovers. We have examined these molecules from the viewpoint of their potential utility in nanoconstruction. Whereas the parallel double helical molecules are usually not well behaved, we have focused on the antiparallel molecules; antiparallel molecules with an even number of half turns between crossovers (termed DAE molecules) produce a reporter strand when ligated, so these have been characterized in a ligation cyclization assay. In contrast to other molecules that contain branched junctions, we find that these molecules cyclize rarely or not at all. The double crossover molecules cyclize no more readily than the linear molecule containing the same sequence as the ligation domain. We have tested both a conventional DAE molecule and one containing a bulged three-arm branched junction between the crossovers. The conventional DAE molecule appears to be slightly stiffer, but so few cyclic products are obtained in either case that quantitative comparisons are not possible. Thus, it appears that these molecules may be able to serve as the rigid components that are needed to assemble symmetric molecular structures, such as periodic lattices. We suggest that they be combined with DNA triangles and deltahedra in order to accomplish this goal. KW - origami KW - origami-design AU - Li, X AU - Yang, X AU - Qi, J AU - Seeman, NC PY - 1996/07/03/ UR - http://dx.doi.org/10.1021/ja960162o ER -