Site-directed modification of DNA duplexes by chemical ligation.

@article{citeulike:2789632, abstract = {The efficiency of chemical ligation method have been demonstrated by assembling a number of DNA duplexes with modified sugar phosphate backbone. Condensation on a tetradecanucleotide template of hexa(penta)- and undecanucleotides differing only in the terminal nucleoside residue have been performed using water-soluble carbodiimide as a condensing agent. As was shown by comparing the efficiency of chemical ligation of single-strand breaks in those duplexes, the reaction rate rises 70 or 45 times if the 3'-OH group is substituted with an amino or phosphate group (the yield of products with a phosphoramidate or pyrophosphate bond is 96-100\% in 6 d). Changes in the conformation of reacting groups caused by mismatched base pairs (A.A, A.C) as well as the hybrid rU.dA pair or an unpaired base make the template-directed condensation less effective. The thermal stability of DNA duplexes was assayed before and after the chemical ligation. Among all of the modified duplexes, only the duplex containing 3'-rU in the nick was found to be a substrate of T4 DNA ligase.}, address = {Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.}, author = {Dolinnaya, N. G. and Sokolova, N. I. and Gryaznova, O. I. and Shabarova, Z. A. }, citeulike-article-id = {2789632}, issn = {0305-1048}, journal = {Nucleic acids research}, keywords = {ligation, origami}, month = {May}, number = {9}, pages = {3721--3738}, posted-at = {2008-05-12 15:44:06}, priority = {2}, title = {Site-directed modification of DNA duplexes by chemical ligation.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3375071}, volume = {16}, year = {1988} }

TY - JOUR ID - citeulike:2789632 L3 - citeulike-article-id:2789632 TI - Site-directed modification of DNA duplexes by chemical ligation. JF - Nucleic acids research VL - 16 IS - 9 SP - 3721 EP - 3738 AD - Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR. SN - 0305-1048 N2 - The efficiency of chemical ligation method have been demonstrated by assembling a number of DNA duplexes with modified sugar phosphate backbone. Condensation on a tetradecanucleotide template of hexa(penta)- and undecanucleotides differing only in the terminal nucleoside residue have been performed using water-soluble carbodiimide as a condensing agent. As was shown by comparing the efficiency of chemical ligation of single-strand breaks in those duplexes, the reaction rate rises 70 or 45 times if the 3'-OH group is substituted with an amino or phosphate group (the yield of products with a phosphoramidate or pyrophosphate bond is 96-100% in 6 d). Changes in the conformation of reacting groups caused by mismatched base pairs (A.A, A.C) as well as the hybrid rU.dA pair or an unpaired base make the template-directed condensation less effective. The thermal stability of DNA duplexes was assayed before and after the chemical ligation. Among all of the modified duplexes, only the duplex containing 3'-rU in the nick was found to be a substrate of T4 DNA ligase. KW - ligation KW - origami AU - Dolinnaya, NG AU - Sokolova, NI AU - Gryaznova, OI AU - Shabarova, ZA PY - 1988/05/11/ UR - http://view.ncbi.nlm.nih.gov/pubmed/3375071 ER -