Sturdier DNA Nanotubes via Ligation

@article{Rothemund2006ligation, abstract = {Abstract: DNA nanotubes are crystalline self-assemblies of DNA tiles ~10 nm in diameter that readily grow tens of micrometers in length. Easy assembly, programmability, and stiffness make them interesting for many applications, but DNA nanotubes begin to melt at temperatures below 40 C, break open when deposited on mica or scanned by AFM, and disintegrate in deionized water. These weaknesses can be traced to the presence of discontinuities in the phosphate backbone, called nicks. The nanotubes studied here have five nicks, one in the core of a tile and one at each corner. We report the successful ligation of all four corner nicks by T4 DNA ligase. Although ligation does not change the nanotubes' stiffness, ligated nanotubes withstand temperatures over 70 C, resist breaking during AFM, and are stable in pure water for over a month. Ligated DNA nanotubes are thus physically and chemically sturdy enough to withstand the manipulations necessary for many technological applications.}, address = {Department of Physics, University of California, Santa Barbara, California 93106, Department of Computer Science and Computational and Neural Systems, California Institute of Technology, Pasadena, California 91125, and Biomolecular Science \& Engineering Program, University of California, Santa Barbara, California 93106}, author = {O'Neill, P. and Rothemund, P. W. K. and Kumar, A. and Fygenson, D. K. }, citeulike-article-id = {1704477}, doi = {10.1021/nl0603505}, journal = {Nano Lett.}, keywords = {background, ligation, lit-sem-2, nanotubes, origami}, month = {July}, number = {7}, pages = {1379--1383}, posted-at = {2007-09-28 10:54:07}, priority = {2}, title = {Sturdier DNA Nanotubes via Ligation}, url = {http://dx.doi.org/10.1021/nl0603505}, volume = {6}, year = {2006} }

TY - JOUR ID - Rothemund2006ligation L3 - citeulike-article-id:1704477 TI - Sturdier DNA Nanotubes via Ligation JF - Nano Lett. VL - 6 IS - 7 SP - 1379 EP - 1383 AD - Department of Physics, University of California, Santa Barbara, California 93106, Department of Computer Science and Computational and Neural Systems, California Institute of Technology, Pasadena, California 91125, and Biomolecular Science & Engineering Program, University of California, Santa Barbara, California 93106 N2 - Abstract: DNA nanotubes are crystalline self-assemblies of DNA tiles ~10 nm in diameter that readily grow tens of micrometers in length. Easy assembly, programmability, and stiffness make them interesting for many applications, but DNA nanotubes begin to melt at temperatures below 40 C, break open when deposited on mica or scanned by AFM, and disintegrate in deionized water. These weaknesses can be traced to the presence of discontinuities in the phosphate backbone, called nicks. The nanotubes studied here have five nicks, one in the core of a tile and one at each corner. We report the successful ligation of all four corner nicks by T4 DNA ligase. Although ligation does not change the nanotubes' stiffness, ligated nanotubes withstand temperatures over 70 C, resist breaking during AFM, and are stable in pure water for over a month. Ligated DNA nanotubes are thus physically and chemically sturdy enough to withstand the manipulations necessary for many technological applications. KW - background KW - ligation KW - lit-sem-2 KW - nanotubes KW - origami AU - O'Neill, P AU - Rothemund, PWK AU - Kumar, A AU - Fygenson, DK PY - 2006/07/12/ UR - http://dx.doi.org/10.1021/nl0603505 ER -