Sequential contribution of L- and P-selectin to leukocyte rolling in vivo.

@article{citeulike:397227, abstract = {Leukocyte recruitment into inflammatory sites is initiated by a reversible transient adhesive contact with the endothelium called leukocyte rolling, which is thought to be mediated by the selectin family of adhesion molecules. Selectin-mediated rolling precedes inflammatory cell emigration, which is significantly impaired in both P- and L-selectin gene-deficient mice. We report here that approximately 13\% of all leukocytes passing venules of the cremaster muscle of wild-type mice roll along the endothelium at < 20 min after surgical dissection. Rolling leukocyte flux fraction reaches a maximum of 28\% at 40-60 min and returns to 13\% at 80-120 min. In P-selectin-deficient mice, rolling is absent initially and reaches 5\% at 80-120 min. Rolling flux fraction in L-selectin-deficient mice is similar to wild type initially and declines to 5\% at 80-120 min. In both wild-type and L-selectin-deficient mice, initial leukocyte rolling (0-60 min) is completely blocked by the P-selectin monoclonal antibody (mAb) RB40.34, but unaffected by L-selectin mAb MEL-14. Conversely, rolling at later time points (60-120 min) is inhibited by mAb MEL-14 but not by mAb RB40.34. After treatment with tumor necrosis factor (TNF)-alpha for 2 h, approximately 24\% of all passing leukocytes roll in cremaster venules of wild-type and P-selectin gene-deficient mice. Rolling in TNF-alpha-treated mice is unaffected by P-selectin mAb or E-selectin mAb 10E9.6. By contrast, rolling in TNF-alpha-treated P-selectin-deficient mice is completely blocked by L-selectin mAb. These data show that P-selectin is important during the initial induction of leukocyte rolling after tissue trauma. At later time points and in TNF-alpha-treated preparations, rolling is largely L-selectin dependent. Under the conditions tested, we are unable to find evidence for involvement of E-selectin in leukocyte rolling in mice.}, address = {Department of Biomedical Engineering, University of Virginia Medical School, Charlottesville 22908.}, author = {Ley, K. and Bullard, D. C. and Arbon\'{e}s, M. L. and Bosse, R. and Vestweber, D. and Tedder, T. F. and Beaudet, A. L. }, citeulike-article-id = {397227}, issn = {0022-1007}, journal = {J Exp Med}, keywords = {leukocyte, p-selectin, rolling}, month = {February}, number = {2}, pages = {669--675}, posted-at = {2005-11-16 17:23:34}, priority = {2}, title = {Sequential contribution of L- and P-selectin to leukocyte rolling in vivo.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7530761}, volume = {181}, year = {1995} }

TY - JOUR ID - citeulike:397227 L3 - citeulike-article-id:397227 TI - Sequential contribution of L- and P-selectin to leukocyte rolling in vivo. JF - J Exp Med VL - 181 IS - 2 SP - 669 EP - 675 AD - Department of Biomedical Engineering, University of Virginia Medical School, Charlottesville 22908. SN - 0022-1007 N2 - Leukocyte recruitment into inflammatory sites is initiated by a reversible transient adhesive contact with the endothelium called leukocyte rolling, which is thought to be mediated by the selectin family of adhesion molecules. Selectin-mediated rolling precedes inflammatory cell emigration, which is significantly impaired in both P- and L-selectin gene-deficient mice. We report here that approximately 13% of all leukocytes passing venules of the cremaster muscle of wild-type mice roll along the endothelium at < 20 min after surgical dissection. Rolling leukocyte flux fraction reaches a maximum of 28% at 40-60 min and returns to 13% at 80-120 min. In P-selectin-deficient mice, rolling is absent initially and reaches 5% at 80-120 min. Rolling flux fraction in L-selectin-deficient mice is similar to wild type initially and declines to 5% at 80-120 min. In both wild-type and L-selectin-deficient mice, initial leukocyte rolling (0-60 min) is completely blocked by the P-selectin monoclonal antibody (mAb) RB40.34, but unaffected by L-selectin mAb MEL-14. Conversely, rolling at later time points (60-120 min) is inhibited by mAb MEL-14 but not by mAb RB40.34. After treatment with tumor necrosis factor (TNF)-alpha for 2 h, approximately 24% of all passing leukocytes roll in cremaster venules of wild-type and P-selectin gene-deficient mice. Rolling in TNF-alpha-treated mice is unaffected by P-selectin mAb or E-selectin mAb 10E9.6. By contrast, rolling in TNF-alpha-treated P-selectin-deficient mice is completely blocked by L-selectin mAb. These data show that P-selectin is important during the initial induction of leukocyte rolling after tissue trauma. At later time points and in TNF-alpha-treated preparations, rolling is largely L-selectin dependent. Under the conditions tested, we are unable to find evidence for involvement of E-selectin in leukocyte rolling in mice. KW - leukocyte KW - p-selectin KW - rolling AU - Ley, K AU - Bullard, DC AU - Arbonés, ML AU - Bosse, R AU - Vestweber, D AU - Tedder, TF AU - Beaudet, AL PY - 1995/02/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/7530761 ER -