EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences.

@article{citeulike:3040748, abstract = {16S rRNA gene sequences have been widely used for the identification of prokaryotes. However, the flood of sequences of non-type strains and the lack of a peer-reviewed database for 16S rRNA gene sequences of type strains have made routine identification of isolates difficult and labour-intensive. In the present study, we generated a database containing 16S rRNA gene sequences of all prokaryotic type strains. In addition, a web-based tool, named EzTaxon, for analysis of 16S rRNA gene sequences was constructed to achieve identification of isolates based on pairwise nucleotide similarity values and phylogenetic inference methods. The system developed provides users with a similarity-based search, multiple sequence alignment and various phylogenetic analyses. All of these functions together with the 16S rRNA gene sequence database of type strains can be successfully used for automated and reliable identification of prokaryotic isolates. The EzTaxon server is freely accessible over the Internet at http://www.eztaxon.org/}, address = {Interdisciplinary Program in Bioinformatics, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea. jchun@snu.ac.kr}, author = {Chun, J. and Lee, J. H. and Jung, Y. and Kim, M. and Kim, S. and Kim, B. K. and Lim, Y. W. }, citeulike-article-id = {3040748}, doi = {10.1099/ijs.0.64915-0}, issn = {1466-5026}, journal = {International journal of systematic and evolutionary microbiology}, keywords = {db, metagenomics, rna}, month = {October}, number = {Pt 10}, pages = {2259--2261}, posted-at = {2008-07-24 18:25:38}, priority = {2}, title = {EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences.}, url = {http://dx.doi.org/10.1099/ijs.0.64915-0}, volume = {57}, year = {2007} }

TY - JOUR ID - citeulike:3040748 L3 - citeulike-article-id:3040748 TI - EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. JF - International journal of systematic and evolutionary microbiology VL - 57 IS - Pt 10 SP - 2259 EP - 2261 AD - Interdisciplinary Program in Bioinformatics, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea. jchun@snu.ac.kr SN - 1466-5026 N2 - 16S rRNA gene sequences have been widely used for the identification of prokaryotes. However, the flood of sequences of non-type strains and the lack of a peer-reviewed database for 16S rRNA gene sequences of type strains have made routine identification of isolates difficult and labour-intensive. In the present study, we generated a database containing 16S rRNA gene sequences of all prokaryotic type strains. In addition, a web-based tool, named EzTaxon, for analysis of 16S rRNA gene sequences was constructed to achieve identification of isolates based on pairwise nucleotide similarity values and phylogenetic inference methods. The system developed provides users with a similarity-based search, multiple sequence alignment and various phylogenetic analyses. All of these functions together with the 16S rRNA gene sequence database of type strains can be successfully used for automated and reliable identification of prokaryotic isolates. The EzTaxon server is freely accessible over the Internet at http://www.eztaxon.org/ KW - db KW - metagenomics KW - rna AU - Chun, J AU - Lee, JH AU - Jung, Y AU - Kim, M AU - Kim, S AU - Kim, BK AU - Lim, YW PY - 2007/10// UR - http://dx.doi.org/10.1099/ijs.0.64915-0 ER -