A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing.

@article{citeulike:1432454, abstract = {MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.}, address = {Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Box 186, The Rockefeller University, New York, NY 10021, USA.}, author = {Landgraf, P. and Rusu, M. and Sheridan, R. and Sewer, A. and Iovino, N. and Aravin, A. and Pfeffer, S. and Rice, A. and Kamphorst, A. O. and Landthaler, M. and Lin, C. and Socci, N. D. and Hermida, L. and Fulci, V. and Chiaretti, S. and Fo\`{a}, R. and Schliwka, J. and Fuchs, U. and Novosel, A. and M\"{u}ller, R. U. and Schermer, B. and Bissels, U. and Inman, J. and Phan, Q. and Chien, M. and Weir, D. B. and Choksi, R. and De Vita, G. and Frezzetti, D. and Trompeter, H. I. and Hornung, V. and Teng, G. and Hartmann, G. and Palkovits, M. and Di Lauro, R. and Wernet, P. and Macino, G. and Rogler, C. E. and Nagle, J. W. and Ju, J. and Papavasiliou, F. N. and Benzing, T. and Lichter, P. and Tam, W. and Brownstein, M. J. and Bosio, A. and Borkhardt, A. and Russo, J. J. and Sander, C. and Zavolan, M. and Tuschl, T. }, citeulike-article-id = {1432454}, doi = {10.1016/j.cell.2007.04.040}, issn = {0092-8674}, journal = {Cell}, keywords = {atlas, bayesian, cloning, expression, mirna, quantitation}, month = {June}, number = {7}, pages = {1401--1414}, posted-at = {2008-07-08 18:50:39}, priority = {2}, title = {A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing.}, url = {http://dx.doi.org/10.1016/j.cell.2007.04.040}, volume = {129}, year = {2007} }

TY - JOUR ID - citeulike:1432454 L3 - citeulike-article-id:1432454 TI - A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing. JF - Cell VL - 129 IS - 7 SP - 1401 EP - 1414 AD - Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Box 186, The Rockefeller University, New York, NY 10021, USA. SN - 0092-8674 N2 - MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses. KW - atlas KW - bayesian KW - cloning KW - expression KW - mirna KW - quantitation AU - Landgraf, P AU - Rusu, M AU - Sheridan, R AU - Sewer, A AU - Iovino, N AU - Aravin, A AU - Pfeffer, S AU - Rice, A AU - Kamphorst, AO AU - Landthaler, M AU - Lin, C AU - Socci, ND AU - Hermida, L AU - Fulci, V AU - Chiaretti, S AU - Foà, R AU - Schliwka, J AU - Fuchs, U AU - Novosel, A AU - Müller, RU AU - Schermer, B AU - Bissels, U AU - Inman, J AU - Phan, Q AU - Chien, M AU - Weir, DB AU - Choksi, R AU - De Vita, G AU - Frezzetti, D AU - Trompeter, HI AU - Hornung, V AU - Teng, G AU - Hartmann, G AU - Palkovits, M AU - Di Lauro, R AU - Wernet, P AU - Macino, G AU - Rogler, CE AU - Nagle, JW AU - Ju, J AU - Papavasiliou, FN AU - Benzing, T AU - Lichter, P AU - Tam, W AU - Brownstein, MJ AU - Bosio, A AU - Borkhardt, A AU - Russo, JJ AU - Sander, C AU - Zavolan, M AU - Tuschl, T PY - 2007/06/29/ UR - http://dx.doi.org/10.1016/j.cell.2007.04.040 ER -