MicroRNA miR-124 regulates neurite outgrowth during neuronal differentiation

@article{citeulike:3084552, abstract = {MicroRNAs (miRNAs) are small RNAs with diverse regulatory roles. The miR-124 miRNA is expressed in neurons in the developing and adult nervous system. Here we show that overexpression of miR-124 in differentiating mouse P19 cells promotes neurite outgrowth, while blocking miR-124 function delays neurite outgrowth and decreases acetylated [alpha]-tubulin. Altered neurite outgrowth also was observed in mouse primary cortical neurons when miR-124 expression was increased, or when miR-124 function was blocked. In uncommitted P19 cells, miR-124 expression led to disruption of actin filaments and stabilization of microtubules. Expression of miR-124 also decreased Cdc42 protein and affected the subcellular localization of Rac1, suggesting that miR-124 may act in part via alterations to members of the Rho GTPase family. Furthermore, constitutively active Cdc42 or Rac1 attenuated neurite outgrowth promoted by miR-124. To obtain a broader perspective, we identified mRNAs downregulated by miR-124 in P19 cells using microarrays. mRNAs for proteins involved in cytoskeletal regulation were enriched among mRNAs downregulated by miR-124. A miR-124 variant with an additional 5' base failed to promote neurite outgrowth and downregulated substantially different mRNAs. These results indicate that miR-124 contributes to the control of neurite outgrowth during neuronal differentiation, possibly by regulation of the cytoskeleton.}, author = {Yu, Jenn-Yah and Chung, Kwan-Ho and Deo, Monika and Thompson, Robert C. and Turner, David L. }, citeulike-article-id = {3084552}, doi = {10.1016/j.yexcr.2008.06.002}, journal = {Experimental Cell Research}, keywords = {brain, mir-124, mirna}, posted-at = {2008-08-05 10:17:47}, priority = {2}, title = {MicroRNA miR-124 regulates neurite outgrowth during neuronal differentiation}, url = {http://dx.doi.org/10.1016/j.yexcr.2008.06.002}, volume = {In Press, Corrected Proof} }

TY - JOUR ID - citeulike:3084552 L3 - citeulike-article-id:3084552 TI - MicroRNA miR-124 regulates neurite outgrowth during neuronal differentiation JF - Experimental Cell Research VL - In Press, Corrected Proof N2 - MicroRNAs (miRNAs) are small RNAs with diverse regulatory roles. The miR-124 miRNA is expressed in neurons in the developing and adult nervous system. Here we show that overexpression of miR-124 in differentiating mouse P19 cells promotes neurite outgrowth, while blocking miR-124 function delays neurite outgrowth and decreases acetylated [alpha]-tubulin. Altered neurite outgrowth also was observed in mouse primary cortical neurons when miR-124 expression was increased, or when miR-124 function was blocked. In uncommitted P19 cells, miR-124 expression led to disruption of actin filaments and stabilization of microtubules. Expression of miR-124 also decreased Cdc42 protein and affected the subcellular localization of Rac1, suggesting that miR-124 may act in part via alterations to members of the Rho GTPase family. Furthermore, constitutively active Cdc42 or Rac1 attenuated neurite outgrowth promoted by miR-124. To obtain a broader perspective, we identified mRNAs downregulated by miR-124 in P19 cells using microarrays. mRNAs for proteins involved in cytoskeletal regulation were enriched among mRNAs downregulated by miR-124. A miR-124 variant with an additional 5' base failed to promote neurite outgrowth and downregulated substantially different mRNAs. These results indicate that miR-124 contributes to the control of neurite outgrowth during neuronal differentiation, possibly by regulation of the cytoskeleton. KW - brain KW - mir-124 KW - mirna AU - Yu, Jenn-Yah AU - Chung, Kwan-Ho AU - Deo, Monika AU - Thompson, Robert AU - Turner, David UR - http://dx.doi.org/10.1016/j.yexcr.2008.06.002 ER -