The oocyte-specific methylated region of the U2afbp-rs/U2af1-rs1 gene is dispensable for its imprinted methylation.

@article{citeulike:2051147, abstract = {Imprinted genes harbor discrete regions which are differentially methylated in gametes; usually the final differential methylation patterns in adults are established during embryogenesis through modifications of the initial methylation patterns in gametes. Previous reports have shown that a 200-bp region termed region II within the CpG island of the mouse imprinted U2afbp-rs gene is methylated in oocytes but not in sperm, suggesting that this region is a center for the propagation of methylated CpGs on the maternal allele and is also a candidate for an imprinting control element. To determine whether region II is required for the imprinted methylation of this gene at the endogenous locus, we generated mice carrying a deletion of this region. We herein show that parental methylation differences still exist in the CpG island on the region II-deleted allele. These findings suggest that region II is dispensable for the imprinted methylation of the U2afbp-rs gene.}, address = {Division of DNA Biology and Embryo Engineering, CREST, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Tokyo, Minato-ku, 108-8639, Japan.}, author = {Sunahara, S. and Nakamura, K. and Nakao, K. and Gondo, Y. and Nagata, Y. and Katsuki, M. }, citeulike-article-id = {2051147}, doi = {http://dx.doi.org/10.1006/bbrc.2000.2189}, issn = {0006-291X}, journal = {Biochem Biophys Res Commun}, keywords = {transgene, u2af1-rs1}, month = {February}, number = {2}, pages = {590--595}, posted-at = {2007-12-03 16:39:19}, priority = {3}, title = {The oocyte-specific methylated region of the U2afbp-rs/U2af1-rs1 gene is dispensable for its imprinted methylation.}, url = {http://dx.doi.org/10.1006/bbrc.2000.2189}, volume = {268}, year = {2000} }

TY - JOUR ID - citeulike:2051147 L3 - citeulike-article-id:2051147 TI - The oocyte-specific methylated region of the U2afbp-rs/U2af1-rs1 gene is dispensable for its imprinted methylation. JF - Biochem Biophys Res Commun VL - 268 IS - 2 SP - 590 EP - 595 AD - Division of DNA Biology and Embryo Engineering, CREST, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Tokyo, Minato-ku, 108-8639, Japan. SN - 0006-291X N2 - Imprinted genes harbor discrete regions which are differentially methylated in gametes; usually the final differential methylation patterns in adults are established during embryogenesis through modifications of the initial methylation patterns in gametes. Previous reports have shown that a 200-bp region termed region II within the CpG island of the mouse imprinted U2afbp-rs gene is methylated in oocytes but not in sperm, suggesting that this region is a center for the propagation of methylated CpGs on the maternal allele and is also a candidate for an imprinting control element. To determine whether region II is required for the imprinted methylation of this gene at the endogenous locus, we generated mice carrying a deletion of this region. We herein show that parental methylation differences still exist in the CpG island on the region II-deleted allele. These findings suggest that region II is dispensable for the imprinted methylation of the U2afbp-rs gene. KW - transgene KW - u2af1-rs1 AU - Sunahara, S AU - Nakamura, K AU - Nakao, K AU - Gondo, Y AU - Nagata, Y AU - Katsuki, M PY - 2000/02/16/ UR - http://dx.doi.org/10.1006/bbrc.2000.2189 ER -