Coupling of Rotation and Catalysis in F1-ATPase Revealed by Single-Molecule Imaging and Manipulation

@article{citeulike:1569725, abstract = {Summary F1-ATPase is a rotary molecular motor that proceeds in 120[degree sign] steps, each driven by ATP hydrolysis. How the chemical reactions that occur in three catalytic sites are coupled to mechanical rotation is the central question. Here, we show by high-speed imaging of rotation in single molecules of F1 that phosphate release drives the last 40[degree sign] of the 120[degree sign] step, and that the 40[degree sign] rotation accompanies reduction of the affinity for phosphate. We also show, by single-molecule imaging of a fluorescent ATP analog Cy3-ATP while F1 is forced to rotate slowly, that release of Cy3-ADP occurs at ~240[degree sign] after it is bound as Cy3-ATP at 0[degree sign]. This and other results suggest that the affinity for ADP also decreases with rotation, and thus ADP release contributes part of energy for rotation. Together with previous results, the coupling scheme is now basically complete.}, author = {Adachi, Kengo and Oiwa, Kazuhiro and Nishizaka, Takayuki and Furuike, Shou and Noji, Hiroyuki and Itoh, Hiroyasu and Yoshida, Masasuke and Kinosita}, citeulike-article-id = {1569725}, doi = {10.1016/j.cell.2007.05.020}, journal = {Cell}, keywords = {experiment, f1-atpase, kinetics, motor}, month = {July}, number = {2}, pages = {309--321}, posted-at = {2007-08-22 08:51:53}, priority = {2}, title = {Coupling of Rotation and Catalysis in F1-ATPase Revealed by Single-Molecule Imaging and Manipulation}, url = {http://dx.doi.org/10.1016/j.cell.2007.05.020}, volume = {130}, year = {2007} }

TY - JOUR ID - citeulike:1569725 L3 - citeulike-article-id:1569725 TI - Coupling of Rotation and Catalysis in F1-ATPase Revealed by Single-Molecule Imaging and Manipulation JF - Cell VL - 130 IS - 2 SP - 309 EP - 321 N2 - Summary F1-ATPase is a rotary molecular motor that proceeds in 120[degree sign] steps, each driven by ATP hydrolysis. How the chemical reactions that occur in three catalytic sites are coupled to mechanical rotation is the central question. Here, we show by high-speed imaging of rotation in single molecules of F1 that phosphate release drives the last 40[degree sign] of the 120[degree sign] step, and that the 40[degree sign] rotation accompanies reduction of the affinity for phosphate. We also show, by single-molecule imaging of a fluorescent ATP analog Cy3-ATP while F1 is forced to rotate slowly, that release of Cy3-ADP occurs at ~240[degree sign] after it is bound as Cy3-ATP at 0[degree sign]. This and other results suggest that the affinity for ADP also decreases with rotation, and thus ADP release contributes part of energy for rotation. Together with previous results, the coupling scheme is now basically complete. KW - experiment KW - f1-atpase KW - kinetics KW - motor AU - Adachi, Kengo AU - Oiwa, Kazuhiro AU - Nishizaka, Takayuki AU - Furuike, Shou AU - Noji, Hiroyuki AU - Itoh, Hiroyasu AU - Yoshida, Masasuke AU - Kinosita PY - 2007/07/27/ UR - http://dx.doi.org/10.1016/j.cell.2007.05.020 ER -